5-Aza-2'-deoxycytidine-5'-triphosphate, Sodium salt
For general laboratory use.
Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
Shelf Life: 12 months after date of delivery
Molecular Formula: C8H15N4O13P3 (free acid)
Molecular Weight: 468.14 g/mol (free acid)
Exact Mass: 467.98 g/mol (free acid)
Purity: ≥ 95 % (HPLC)
Color: white to off-white
Spectroscopic Properties: λmax 244 nm, ε 7.0 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Determination of methyltransferase activity
5-Aza-dCTP is a inhibitor of DNA methylation and an antileukemic agent. It has been used to reactivate silent tumor suppressor genes.
1.) Contains at least 10 mg product
2.) The triazine moiety of decitabine is prone to acid- and base-catalyzed decomposition. Always prepare a fresh solution for immediate use. Neutral buffers and low temperatures are most appropriate.
BIOZ Product Citations:
 Frauer et al. (2009) A versatile non-radioactive assay for DNA methyltransferase activity and DNA binding. Nucleic Acids Research 37 (3):e22.
 Lin et al. (1981) High-performance liquid chromatographic analysis of chemical stability of 5-aza-2'-deoxycytidine J. Pharm. Sci. 70 (11):1228.
Lemaire et al. (2005) Enhancement of antineoplastic action of 5-aza-2'-deoxycytidine by zebularine on L1210 leukemia. Anticancer Drugs. 16 (3):301.
Parker et al. (1987) Inhibition of DNA primase by nucleoside triphosphates and their arabinofuranosyl analogs. Mol. Pharmacol. 31 (2):146.
McIntosh et al. (1985) Synthesis and characterization of poly[d (G-z5C)]. B-Z transition and inhibition of DNA methylase. Biochemistry 24 (18):4806.
Momparler et al. (1984) Kinetic interaction of 5-AZA-2'-deoxycytidine-5'-monophosphate and its 5'-triphosphate with deoxycytidylate deaminase. Mol. Pharmacol. 25 (3):436.
Bouchard et al. (1983) Incorporation of 5-Aza-2'-deoxycytidine-5'-triphosphate into DNA. Interactions with mammalian DNA polymerase alpha and DNA methylase. Mol. Pharmacol. 24 (1):109.
Bouchard et al. (1983) Incorporation of 5-Aza-2'-deoxycytidine-5'-triphosphate into DNA. Interactions with mammalian DNA polymerase alpha and DNA methylase. Mol. Pharmacology 24 (1):109.