5-Ethenyl-uridine
| Cat. No. | Amount | Price (EUR) | Buy / Note |
|---|---|---|---|
| CLK-049-10 | 10 mg | 132,70 | Add to Basket/Quote Add to Notepad |
| CLK-049-50 | 50 mg | 457,60 | Add to Basket/Quote Add to Notepad |
For general laboratory use.
Shipping: shipped at ambient temperature
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C11H14N2O6
Molecular Weight: 270.24 g/mol
Exact Mass: 270.09 g/mol
CAS#: 55520-64-4
Purity: ≥ 98 % (HPLC)
Form: solid
Color: white to off-white
Solubility: DMSO, methanol
Spectroscopic Properties: λmax 288 nm, ε 8.5 L mmol-1 cm-1 (Methanol)
Applications:
RNA synthesis monitoring (potentially)
Description:
5-VU (5-Vinyl-uridine) can potentially be used as a replacement for BrU (5-Bromo-uridine) or the copper-catalyst requiring 5-EU (5-Ethynyl-uridine) to measure de novo RNA synthesis in proliferating cells. 5-VU is cell permeable and might incorporate into nascent RNA instead of its natural analog uridine. This assumption is based on the previously demonstrated suitability of 5-VdU (5-Vinyl-2'-deoxyuridine) for DNA synthesis monitoring[1].
The resulting vinyl-functionalized RNA could subsequently be detected via Cu(I)-free Alkene-Tetrazine Ligation that offers the choice to introduce a Biotin group (via Tetrazines of Biotin) for subsequent purification tasks or a fluorescent group (via Tetrazines of fluorescent dyes) for subsequent microscopic imaging.
Related products: 5-Ethynyl-uridine (5-EU), #CLK-N002
BIOZ Product Citations:
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Selected References:
[1] Rieder et al. (2014) Alkene-Tetrazine Ligation for Imaging Cellular DNA. Angew. Chem. Int. Ed. 53:9168.
Liu et al. (2019) A Nucleoside Derivative 5-Vinyluridine (VrU) for Imaging RNA in Cells and Animals. Bioconjug. Chem. 30 (11):2958.
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