For research use only!
Shipping: shipped at ambient temperature
Storage Conditions: store at -20 °C
store dry and under inert gas
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C11H13N3O5
Molecular Weight: 267.24 g/mol
Exact Mass: 267.09 g/mol
Purity: ≥ 99 % (HPLC)
Color: white to off-white
Spectroscopic Properties: λmax 294 nm, ε 8.5 L mmol-1 cm-1 (Tris-HCl pH 7.5)
RNA synthesis monitoring
Ethynyl-labeled cytidine (5-EC) can be used as a replacement for BrU (5-Bromo-uridine) to measure de novo RNA synthesis in proliferating cells. 5-EC is cell permeable and incorporates into nascent RNA instead of its natural analog cytidine.
The resulting ethynyl-functionalized RNA can subsequently be detected via Cu(I)-catalyzed click chemistry that offers the choice to introduce a Biotin group (via Azides of Biotin) for subsequent purification tasks or a fluorescent group (via Azides of fluorescent dyes) for subsequent microscopic imaging.
Presolski et al. and Hong et al. provide a general protocol for Cu(I)-catalyzed click chemistry reactions that may be used as a starting point for the set up and optimization of individual assays.
 Qu et al. (2013) 5-Ethynylcytidine as a new agent for detecting RNA synthesis in live cells by click chemistry. Anal. Biochem. 434:128.