UTP - Solution

For general laboratory use.

Please centrifuge briefly before opening (volume ≤2 ml).

Description:
Ultrapure UTP supplied as clear aqueous solution.

Shipping: shipped on gel packs

Storage Conditions: store at -20 °C

Shelf Life: 12 months

Molecular Formula: C₉H₁₅N₂O₁₅P₃ (free acid)

Molecular Weight: 484.14 g/mol (free acid)

CAS#: 19817-92-6

Purity: ≥ 99 % (HPLC)

Form: clear aqueous solution

Concentration: 100 mM ±2 %

pH: 8.0 ±0.2 (22 °C)

Spectroscopic Properties: λ_max 262 nm, ε 9.8 L mmol^-1 cm^-1 (Tris-HCl pH 7.0)

Applications:
Activation of purinergic receptors^[1,2,3,4]
Cardioprotection against hypoxic damage^[2]
Enzyme kinetic parameters^[5]
Phosphorylation of EGF-receptor vie purinergic receptors^[3]
Stimulation of neurogenesis and dopaminergic neurons^[6]

Specific Ligands:

Enterovirus 71 3D RNA polymerase^[7]

Ligand for purinergic receptors:
P2X₁^[4]
P2Y₂^[7,8,9,10]
P2Y₄^[8,10,11]
P2Y₆^[8]

Selected References:
[1] Raqeeb et al. (2011) Purinergic P2Y2 receptors mediate rapid Ca2+ mobilization, membrane hyperpolarization and nitric oxide production in human vascular endothelial cells. Cell Calcium 49:240.
[2] Golan et al. (2011) Extracellular nucleotide derivatives protect cardiomyocytes against hypoxic stress. Biochemical Pharmacology 81:1219.
[3] Boucher et al. (2011) Distinct activation of epidermal growth factor receptor by UTP contributes to epithelial cell wound repair. American Journal Pathology 178:1092.
[4] Sugihara et al. (2011) Dual signaling pathway of arterial constriction by extracellular uridine-5-triphosphate in the rat. J. Pharmacological Sciences (Japan) 115:293.
[5] Ma et al. (2011) Molecular cloning and analysis of the UDP-glucose pyrophosphorylase in Streptococcus equi subsp. Zooepidemicus. Molecular Biology Reports 38:2751.
[6] Delic et al. (2011) Nucleotides affect neurogenesis and dopaminergic differentiation of mouse fetal midbrain-derived neural precursor cells. Puronergic Signalling 6:417.
[7] Jiang et al. (2011) Biochemical characterization of enterovirus 71 3D RNA polymerase. Biochim. Biophys. Acta, Gene Regulatory Mechanisms 1809:211.
[8] Pendergast et al. (2001) Synthesis and P2Y receptor activity of a series of uridine dinucleoside 5'-polyphosphates. Bioorg. Med. Chem. Lett. 11 (2):157.
[9] Shaver et al. (1997) 4-substituted uridine 5'-triphosphates as agonists of the P2Y2 purinergic receptor. Nucleosides and Nucleotides 16 (7):1099.
[10] Kim et al. (2002) Methanocarba modification of uracil and adenine nucleotides: High potency of northern ring conformation at P2Y1, P2Y2, P2Y4 and P2Y11 but not P2Y6 receptors. J. Med. Chem. 45:208.
[11] Nguyen et al. (1995) Cloning, expression, and chromosomal localization of human uridine nucleotide receptors. J. Biol. Chem. 270 (52):30845.