Proteinase K - Solution

For general laboratory use.

Please centrifuge briefly before opening (volume ≤2 ml).

Description:
Proteinase K is a serine protease that exhibits a very broad cleavage specificity. The Protein with a molecular weight of 28.9 kDa cleaves peptide bonds adjacent to the carboxylic group of aliphatic and aromatic amino acids. Proteinse K is not inactivated by metal chelating reagents such as EDTA or detergents such as SDS and is active over a wide range of pH (4 - 12.5).
Proteinase K is a highly active and stable protease with low cutting specificity. The enzyme belongs to the group of subtilisine-related serine proteases and is strongly inhibited by PMSF.
In presence of 0.5 - 1 % SDS Proteinase K inactivates DNases and RNases in eucaryotic and microbiological cell cultures. The use of Proteinase K during lysis of the cells allows the isolation of intact highly-molecular nucleic acids.

Unit Definition: One unit is defined as the amount of enzyme required to hydrolyze casein to produce 1 μmol tyrosine per minute at 37°C and pH 8.0.

Shipping: shipped on gel packs

Storage Conditions: store at -20 °C
avoid freeze/thaw cycles

Shelf Life: 12 months

Molecular Weight: 28.9 kDa

CAS#: 39450-01-6

EC number: 254-457-8

Purity: free of RNases and DNases

Form: Proteinase K solution (20 mg/ml) in 10 mM Tris-HCl, 1 mM Ca(H₃C₂O₂)₂, 10 % (v/v) Glycerol, pH 7.8 (22°C)

Concentration: 20 mg/ml

Activity:
> 600 units/ml

Applications:
Digestion of proteins during DNA and RNA preparation.

Hazard pictograms:

Signal word: Danger

Hazard statements:
H315 Causes skin irritation.
H319 Causes serious eye irritation.
H334 May cause allergy or asthma symptoms or breathing difficulties if inhaled.

Precautionary statements:
P280 Wear protective gloves/protective clothing/eye protection/face protection/hearing protection/....
P302 + P352 IF ON SKIN: Wash with plenty of water/....
P304 + P340 IF INHALED: Remove person to fresh air and keep comfortable for breathing.
P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present
and easy to do. Continue rinsing.
P337 + P313 If eye irritation persists: Get medical advice/attention.
P362 + P364 Take off contaminated clothing and wash it before reuse.