SDS PAGE Sample Buffer - 2x conc.
For research use only.
Please centrifuge briefly before opening (volume ≤2 ml).
Description:
SDS PAGE Sample Buffer is the most commonly used sample buffer for Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis (SDS-PAGE) of denatured proteins. SDS PAGE Sample Buffer ensures optimal band resolution when preparing proteins for SDS-PAGE with Tris-glycine-SDS running buffer.
Shipping: shipped at ambient temperature
Storage Conditions: store at 4 °C
Shelf Life: 12 months
Form: liquid
Concentration: 2x
Content:
2x concentration of SDS PAGE Sample Buffer contains:
0.15 M Tris-HCl pH 6.8
10 % Mercaptoethanol
1.2 % SDS
30 % Glycerol
0.04 % Bromphenol blue
Quality:
- Molecular biology grade
This mixture contains dangerous substances
Signal word: Danger
Hazard statements
H302 + H312 + H332 Harmful if swallowed, in contact with skin and if inhaled.
H410 Very toxic to aquatic life with long lasting effects.
Precautionary statements
P261 Avoid breathing dust/fume/gas/mist/vapours/spray.
P273 Avoid release to the environment.
P280 Wear protective gloves/protective clothing/eye protection/face protection.
P301 + P312 IF SWALLOWED: Call a POISON CENTER/doctor/./ if you feel unwell.
P302 + P352 IF ON SKIN: Wash with plenty of soap and water.
P304 + P340 IF INHALED: Remove person to fresh air and keep comfortable for breathing.
For further information see Material Safety Data Sheet.
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Scientific citations for Jena Bioscience bu-126
7 scientific citations and 1 citation images found by Bioz.
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Picornavirus Evolution: Genomes Encoding Multiple 2A NPGP Sequences—Biomedical and Biotechnological Utility
Viruses, 2024-10-09 · PMID:39459920
.. using nuclease-free water.The final reaction volume was adjusted to 25 μL using nuclease-free water.. Reactions were incubated at
30 °C for90 min before the addition of2x SDS-PAGE loading buffer (Jena Bioscience , Jena, Germany).. Translation products were analysed by 4–12% gradient SDS-PAGE (NuPAGE, Invitrogen, Paisley, UK) and the .. -
Timeless couples G‐quadruplex detection with processing by DDX 11 helicase during DNA replication
The EMBO Journal, 2020-07-23 · PMID:32705708
Samples were quantified using the Bradford method, and approximately 30 μg were mixed with 5×
SDS–Page Sample Buffer (Jena Bioscience ), boiled for 3 min, fractionated in 4–12 or 10% Nu‐PAGE Bis‐Tris gels (Thermo Fisher Scientific) in MOPS (200 mM MOP, 50 mM Sodium Acetate, 10 mM Na 2 EDTA) and transferred to nitrocellulose membranes using an iBlot2 apparatus (Thermo Fisher Scientific) at 25 V for 7 min. Membranes were blocked for 1 h at room temperature with TBS 0.1% Tween‐20 5% skimmed milk. -
CryoEM structures of human CMG–ATPγS–DNA and CMG–AND-1 complexes
Nucleic Acids Research, 2020-07-09 · PMID:32453425
.. purification protocol was followed until the StrepTrap column loading step.. After sample loading, the column was washed for 5 column volumes using Buffer N with 2 mM DTT, followed by 5 column volumes of
Buffer S . To form the CMG–ATPγS–DNA complex, ∼900 μl of 4 μM forked DNA duplex and 100 μM ATPγS (Jena Bioscience , #NU-406-50) in Buffer S was applied to the column at 0.05 ml/min.. The column was first washed in reverse flow for 4 ..
