Crystallization grade model protein
Mucopeptide N-acetylmuramoyl-hydrolase, Muramidase
For in vitro use only!
Shipping: shipped at ambient temperature
Storage Conditions: store at 4 °C
do not freeze
Shelf Life: 12 months
Molecular Weight: 14.3 kDa (single chain)
EC number: 235-747-3
Form: aqueous solution
Concentration: 20 mg/ml
pH: 3.5 (H2O, 20 °C)
Solubility: 300 g/l in water
Activity: 50 kU/mg (Micrococcus luteus, FIP-Standard; pH 7.0; 25 °C)
Lysozyme can be utilized as model protein in crystallization experiments and X-ray structure analysis.
Lysozyme is a glycosidase which hydrolyzes the ß 1,4 glycosidic bond between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in the bacterial peptidoglycan.
Source: Chicken egg white
Crystals can be grown using the sitting drop, hanging drop or microbatch method.
Following crystallization conditions are suggested for lysozyme crystal growth:
BIOZ Product Citations:
Please click the arrow on the right to expand the citation list. Click publication title for the full text.
 Rye et al. (2000) Glycosidase mechanisms. Curr. Opin. Chem. Biol. 4:573.
 Gilliland et al. (2002) The Biological Macromolecule Crystallization Database: crystallization procedures and strategies. Acta Cryst. D 58:916.