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Vinyl-containing Nucleosides

Vinyl-containing nucleosides can be labeled with Tetrazine-tagged reporter molecules via Copper-free Tetrazine-Alkene Ligation that allows to introduce a Biotin group for subsequent purification (via Tetrazine-containing Biotinylation Reagents) or a fluorescent group for subsequent microscopic imaging (via Tetrazine-containing Fluorescent Dyes).

They are suitable for DNA synthesis monitoring or RNA synthesis monitoring, respectively.