For research use only!
Shipping: shipped at ambient temperature
Storage Conditions: store at -20 °C
Shelf Life: 12 months
Molecular Formula: C12H21N3O10
Molecular Weight: 367.31 g/mol (free acid)
Purity: ≥ 90 % (TLC)
Form: white to off-white solid
Solubility: water, DMSO
Metabolic Lipopolysaccharide labeling of Myobacteria
Myobacteria are characterized by a trehalose-lipopolysaccharide-containing outer cell membrane.
The Azide-functionalized 6-Azido-Trehalose provides an attractive approach for the detection of Myobacteria in living cultures: It is cell-permeable, intracellularly processed and incorporated instead of its natural counterpart.
The resulting Azide-functionalized lipopolysaccharides can subsequently be detected via Cu(I)-catalyzed or Cu(I)-free Click Chemistry that offers the choice to introduce a Biotin group (via Alkynes of Biotin or DBCO-containing Biotin, respectively) for subsequent purification tasks or to introduce fluorescent group (via Alkynes of fluorescent dyes or DBCO-containing fluorescent dyes, respectively) for subsequent microscopic imaging.
Myobacteria strains tested:
Mycobacterium smegmatis mc2155, Mycobacterium tuberculosis H37Rv,Mycobacterium bovis BCG
 Swarts et al. (2012) Probing the Mycobacterial Trehalome with Bioorthogonal Chemistry. J. Am. Chem. Soc. 134 (39):16123.