8-Azido-ATP

For general laboratory use.

Shipping: shipped on gel packs

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Shelf Life: 12 months after date of delivery

Molecular Formula: C₁₀H₁₅N₈O₁₃P₃ (free acid)

Molecular Weight: 548.19 g/mol (free acid)

Exact Mass: 548.00 g/mol (free acid)

Purity: ≥ 95 % (HPLC)

Form: solution in water

Color: colorless to slightly yellow

Concentration: 10 mM - 11 mM

pH: 7.5 ±0.5

Spectroscopic Properties: λ_max 281 nm, ε 13.3 L mmol^-1 cm^-1 (Tris-HCl pH 7.5)

Applications:
Incorporation into RNA by 3'-End Labeling with yeast Poly(A) Polymerase(yPAP)^[1]
Identification of protein ATP-binding sites^[2]
The resulting azide-functionalized RNA can subsequently be processed via Cu(I)-free (azide-DBCO) or Cu(I)-catalyzed (azide-alkyne) click chemistry that offers the choice
- to introduce a Biotin group for subsequent purification tasks (via DBCO-functionalized Biotin or Alkynes of Biotin, respectively)
- to introduce fluorescent group for subsequent microscopic imaging (DBCO-functionalized fluorescent dyes or Alkynes of fluorescent dyes, respectively)
- to crosslink the RNA to azide- or alkyne functionalized biomolecules e.g.proteins

BIOZ Product Citations:

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Selected References:
[1] Chen et al. (2004) Chain Termination and Inhibition of Saccharomyces cerevisiae Poly (A) Polymerase by C-8-modified ATP analogs. J. Biol. Chem. 279 (39):40405.
[2] Olcott et al. (1998) Localization and Characterization of Two Nucleotide-binding Sites on the Anaerobic Ribonucleotide Reductase from Bacteriophage T4. J. Biol. Chem. 273 (38):24853.
[3] Zayas et al. (2015) Strain Promoted Click Chemistry of 2- or 8-Azidopurine and 5-Azidopyrimidine Nucleosides and 8-Azidoadenosine Triphosphate with Cyclooctynes. Application to Living Cell Fluorescent Imaging. Bioconjug Chem. 26 (8):1519-32.