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Fission Impossible: Non-hydrolyzable nucleotide analogs

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To study:

  • protein-protein interactions
  • structure and dynamics of protein-nucleotide-complexes
  • NTPase kinetics
Learn more below!

Crucial cellular processes such as transport, motion and division are orchestrated by a highly diverse set of regulatory phosphatases. To trap these enzymes in their various biologically relevant states, non-hydrolyzable analogs of nucleotides are ideally suited. Jena Bioscience has more than two decades of experience in the preparation of non-hydrolyzable nucleotides, supplying researchers with >50 catalog products (see Scheme 1) of this compound class. At the forefront of science1-10 it is our mission to facilitate your work on:

  • Identification of protein-protein interactions and enzyme substrates
  • Elucidation of structure and dynamics of protein-nucleotide-complexes
  • Kinetic analysis of nucleotide exchange reactions on NTPase

Non-hydrolyzable Nucleotide Kits Non-hydrolyzable Di-Nucleotides beta,gamma non-hydrolyzable Nucleotides alpha,beta non-hydrolyzable Nucleotides alpha-Phosphate modified Nucleotides beta-Phosphate modified Nucleotides gamma-Phosphate modified Nucleotides

Scheme 1: Overview of Jena Bioscience’s non-hydrolyzable nucleotides. Chemical resistance to hydrolysis can be achieved by replacing bridging oxygens for imido (-NH-) or methylene (-CH2-) moieties. Moreover, phosphorothioates show markedly reduced rates of hydrolysis compared to the corresponding phosphate analogues while preserving a high sterical similarity. (Please click the position of modification topic for ordering information.)

Recent research highlights applying Jena Bioscience’s non-hydrolyzable nucleotides


[1] Waschbüsch et al. (2019) Rab32 interacts with SNX6 and affects retromer-dependent Golgi trafficking. PLoS One 14 (1):e0208889.
[2] Wachtel et al. (2018) The protease GtgE from Salmonella exclusively targets inactive Rab GTPases. Nat. Commun. 9 (1):44.


GppNHp (Cat. No. NU-401)

  • Pulldown studies on GppNHp-bound Rab321
  • Preparation various NTP-loaded Rab proteins as substrates for a protease activity assay2

[3] Whitecross et al. (2017) Identification of the Binding Sites on Rab5 and p110beta Phosphatidylinositol 3-kinase. Sci. Rep. 7 (1):16194.


GppCp (Cat. No. NU-402)

  • GST-pulldown studies using GppCp-loaded Rab5 or Rac1

[4] Bodnar et al. (2017) Molecular Mechanism of Substrate Processing by the Cdc48 ATPase Complex. Cell 169 (4):722.


ATPγS (Cat. No. NU-406)

  • Activity and crosslinking studies on the Cdc48 ATPase complex
Structure and Dynamics

[5] Parker et al. (2018) K-Ras Populates Conformational States Differently from Its Isoform H-Ras and Oncogenic Mutant K-RasG12D. Structure 26 (6):810.


Gpp15NHp (Custom Product)

  • NMR-Studies on Gpp15NHp-bound Ras

[6] Talavera et al. (2018) Phosphorylation decelerates conformational dynamics in bacterial translation elongation factors. Sci. Adv. 4 (3):eaap9714.


GppNHp (Cat. No. NU-401)

  • Observation of EF-Tu conformational dynamics spFRET

[7] Biancucci et al. (2018) The bacterial Ras/Rap1 site-specific endopeptidase RRSP cleaves Ras through an atypical mechanism to disrupt Ras-ERK signaling. Sci. Signal. 11 (550). pii: eaat8335.
[8] Lüningschrör et al. (2017) Plekhg5-regulated autophagy of synaptic vesicles reveals a pathogenic mechanism in motoneuron disease. Nat. Commun. 8 (1):678.


Mant-GppNHp (Cat. No. NU-207)

  • Nucleotide exchange assay on KRAS7
  • Stopped-flow measurements of nucleotide exchange8

[9] O'Donnell et al. (2017) Timing and Reset Mechanism of GTP Hydrolysis-Driven Conformational Changes of Atlastin. Structure 25 (7):997.


Mant-GTPγS (Cat. No. NU-209)

  • Kd-, kon- and koff- determination of Mant-GTPγS-binding to ATL GTPases by stopped flow

[10] Yu et al. (2018) Cryo-EM Structures of MDA5-dsRNA Filaments at Different Stages of ATP Hydrolysis. Mol. Cell. 72 (6):999.


Mant-AppNHp (Cat. No. NU-214)

  • Fluorescence polarization assay on the MAD5 ATPase

Get in contact with us!

Dr. Thomas Waldbach

You haven't found the particular nucleotiode analog you're looking for? You have questions or need further information? Do not hesitate to contact us at nucleotides@jenabioscience.com !