recombinant, E. coli overexpressing Saccharomyces cerevisiae Poly(A) Polymerase
For in vitro use only!
Unit Definition: One unit is defined as the amount of the enzyme required to catalyze the incorporation of 1 pmol of AMP into an acid-insoluble form in 1 minutes at 37 °C.
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Purity: ≥ 95 % (SDS-PAGE)
Concentration: 600 units/μl
Yeast Poly(A) Polymerase catalyzes the transfer of AMP to 3’-hydroxyl ends of RNA molecules. The reaction is template-independent, requires ATP as substrate and Mg2+ or Mn2+ as cofactor. It works more efficiently than E. coli Poly(A) polymerase in some poly(A) tailing and RNA labeling reactions (e.g. shorter incubation time, broader acceptance of RNA template size).
Polyadenylation increases (m)RNA stability and therefore translation efficiency in transfection and microinjection experiments in eukaryotic cells.
For information on (m)RNA polyadenylation using in vitro transcription reaction mixes as template, refer to the Poly(A) Tailing Enzyme Testkit (#RNT-004).
Yeast Poly(A) Polymerase
#RNT-006-S: 1x 50 μl (600 units/μl)
#RNT-006-L: 3x 50 μl (600 units/μl)
20 mM Tris-HCl (pH 8.0), 50 mM KCl, 0.5 mM DTT, 50% Glycerol (v/v)
Yeast Poly(A) Polymerase Reaction Buffer
1x 1.2 ml (5x)
100 mM Tris-HCl (pH 7.0), 3 mM MnCl2, 0.1 mM EDTA, 1 mM DTT, 0.5 mg/ml acetylated BSA, 50% glycerol (v/v)
ATP - Solution
1x 100 μl (100 mM)
Related products: PCR-grade water, #PCR-258