Yeast Poly(A) Polymerase
For research use only.
Please centrifuge briefly before opening (volume ≤2 ml).
Description:
Yeast Poly(A) Polymerase catalyzes the transfer of AMP to 3’-hydroxyl ends of RNA molecules. The reaction is template-independent, requires ATP as substrate and Mg2+ or Mn2+ as cofactor. It works more efficiently than E. coli Poly(A) polymerase in some poly(A) tailing and RNA labeling reactions (e.g. shorter incubation time, broader acceptance of RNA template size).
Polyadenylation increases (m)RNA stability and therefore translation efficiency in transfection and microinjection experiments in eukaryotic cells.
Unit Definition: One unit is defined as the amount of the enzyme required to catalyze the incorporation of 1 pmol of AMP into an acid-insoluble form in 1 minutes at 37 °C.
Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Purity: ≥ 95 % (SDS-PAGE)
Form: liquid
Concentration: 600 units/μl
Content:
Yeast Poly(A) Polymerase
#RNT-006-S: 1x 50 μl (600 units/μl)
#RNT-006-L: 3x 50 μl (600 units/μl)
20 mM Tris-HCl (pH 8.0), 50 mM KCl, 0.5 mM DTT, 50% Glycerol (v/v)
Yeast Poly(A) Polymerase Reaction Buffer
1x 1.2 ml (5x)
100 mM Tris-HCl (pH 7.0), 3 mM MnCl2, 0.1 mM EDTA, 1 mM DTT, 0.5 mg/ml acetylated BSA, 50% glycerol (v/v)
ATP - Solution
1x 100 μl (100 mM)
Related products: PCR-grade water, #PCR-258
| Cat. No. | Amount | Price |
|---|---|---|
| RNT-006-S | 30.000 units | 212,20 € |
| RNT-006-L | 3 x 30000 units | 572,90 € |
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Scientific citations for Jena Bioscience rnt-006
11 scientific citations and 0 citation images found by Bioz.
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Primer- and template-independent RNA polymerization by terminal nucleotidyltransferase TENT4B
Nucleic Acids Research, 2026-09-23 · PMID:42703972
.. were stained using Sybr Gold and imaged using the Cy3 channel of the imager.. Reactions with
yeast poly(A) polymerase (YPAP;Jena Biosciences ,RNT-006 -
A kinetic ruler controls mRNA poly(A) tail length.
Genes & Development, 2025-11-03 · PMID:40846642
.. Table S5 for the specification of conditions and final concentrations.. Reactions involv- ing only
yeast poly(A) polymerase (final concentration 35 U/μL;Jena Bioscience RNT-006 ) were performed in poly(A) polymerase reaction buffer (20 mM Tris-HCl at pH 7.0, 0.6 mM MnCl2, 0.02 mM EDTA, 0.02 mM DTT, 0.1 mg/mL acetylated BSA, 10% [v/v] glycerol, 0.1 U/μL RiboLock, 0.5 mM ATP).. Reactions were stopped after 4 min (or at the indicated .. -
High Resolution Dynamic Imaging of Chromatin DNA Communication using Oligo-LiveFISH
Cell, 2025-06-12 · PMID:40239646
.. Zymo Research , Cat#R1018.RNA Clean & Concentrator TM -25 , Zymo Research , Cat#R1018..
yeast poly(A) polymerase ,Jena Bioscience , Cat#RNT-006 -S.. RNase inhibitor , NEB , Cat#M0314S.RNase inhibitor , NEB , Cat#M0314S.
