For in vitro use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Form: liquid (Supplied as serum, preserved in glycerol)
Anti A-Allatostatin can be used for ELISA and Immunocytochemistry.
The anti-allatostatin serum was raised against the Diploptera punctata A-type Dip-allatostatin I, APSGAQRLYGFGL amide, coupled to bovine thyroglobulin using glutaraldehyde (Viztum et.al. 1996) and that previously has been used to localize A-ASTs in insect, crustacean and spider nervous systems
The Dip-AST serum displays no cross-reactivity with corazonin, CCAP, FMRF amide, leucomyosuppression, locustatachykinin II, M1, perisulfakinin, and proctolin as tested by non-competitive ELISA (Viztum et.al. 1996). These antiserum recognized all A-ASTs that share a -YXFGLamide core (Kreissl et al. 2010, Polanska et al. 2012)
Please click the black arrow on the right to expand the citation list. Click publication title for the full text.
Polanska et al. (2012) Neuropeptide complexity in the crustacean central olfactory pathway: immunolocalization of A-type allatostatins and RFamide-like peptides in the brain of a terrestrial hermit crab. Mol Brain.5:29.
Loesel et al. (2011) Neuroarchitecture of the arcuate body in the brain of the spider Cupiennius salei (Araneae, Chelicerata) revealed by allatostatin-, proctolin-, and CCAP-immunocytochemistry and its evolutionary implications. Arthropod Struct Dev. 40:210.
Kreissl et al. (2010) Allatostatin immunoreactivity in the honeybee brain. J. Comp. Neurol. 518:1391.
Vitzthum et al. (1996) Distribution of Dip-allatostatin I-like immunoreactivity in the brain of the locust Schistocerca gregaria with detailed analysis of immunostaining in the central complex. J. Comp. Neurol. 369: 419