anti-Pam-Igloo-L

Fig. 1

Fig. 2

For general laboratory use.

Shipping: shipped on gel packs

Storage Conditions: store at -20 °C
avoid freeze/thaw cycles

Shelf Life: 12 months

Form: liquid (Supplied as serum, preserved in glycerol)

Applications:
Anti-Pam-Igloo-L can be used for ELISA and Immunocytochemistry.

Description:
Axogenesis and synaptic signal transmission in the vertebrate nervous system are based on the abundant expression of the growth-associated protein GAP-43. In non-vertebrates a GAP-43 related gene (igloo, invertebrate GAP-43 like) was found in Drosophila and encodes two proteins, IGLOO-L and IGLOO-S, with sequence homology and similar biochemical activity to GAP-43. We have cloned a GAP-43-like gene in a holometabolic insect, the American cockroach, Periplaneta americana. As for Drosophila, we found two putative pigl mRNAs, pigl-L and pigl-S, in the cockroach (Fig. 1). Immunocytochemical investigations with a antiserum to Pam-Igl L and Pam-Igl S provide a function for Pam-Igloo L in establishment or maintenance of synaptic contacts and can help to understand the ongoing expression of GAP-43 in the mature nervous system (Hänold,2004).

Specificity:
The specificity against P-IGL-L, but not P-IGL-S, was demonstrated by transfected CHO cells, where the antibody selectively recognizes IGL-L. This P-IGL-L-like immunoreactivity (P-IGL-L-li) was colocalized with the fluorescence signal from the IGL-L-EGFP fusion protein.
Furthermore, the antiserum was tested on neuronal tissue sections of the American cockroach: P-IGL-L-li could be blocked by preincubation of the antibody with recombinant P-IGL-L protein. There was also no immunostaining after replacement of antiserum with preimmune serum (Fig 2). The specificity of the antiserum against Periplaneta americana IGL-L was investigated by immunostaining of brain sections from different cockroach species.

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Selected References:
Hänold R, Dissertation, Friedrich-Schiller-Universität Jena (2004) Klonierung, Überexpression und immuncytochemische Lokalisation des axonalen Wachstumsfaktors GAP-43 bei Insekten: Invertebrate GAP-43 like Protein
Hänold et al. (2003) Immunocytochemical localization of IGL, a new GAP-43 like gene product in different developmental stages of the Amercan cockraoach. 29.Goettingen Neurobiology Conference 921,p931 http://nwg.glia.mdcberlin.de/media/pdf/conference/ proceedings (2003)
Neel et al. (1994) Igloo, a GAP-43-related gene expressed in the developing nervous system of Drosophila. Development 120:2235.