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5-DBCO-PEG4-dUTP

5-Dibenzylcyclooctyl-PEG4-uridine-5'-triphosphate, Triethylammonium salt

Cat. No. Amount Price (EUR) Buy / Note
CLK-048-S 50 μl (10 mM) 127,65 Add to Basket/Quote Add to Notepad
CLK-048-L 5 x 50 μl (10 mM) 510,59 Add to Basket/Quote Add to Notepad
Structural formula of 5-DBCO-PEG4-dUTP (5-Dibenzylcyclooctyl-PEG4-uridine-5'-triphosphate, Triethylammonium salt)
Structural formula of 5-DBCO-PEG4-dUTP

For research use only!

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Shelf Life: 12 months after date of delivery

Molecular Formula: C42H54N5O21P3 (free acid)

Molecular Weight: 1057.83 g/mol (free acid)

Purity: ≥ 95 % (HPLC)

Form: clear aqueous solution in 100 mM Tris-HCl

Concentration: 10 mM - 11 mM

pH: 7.5 ±0.5

Spectroscopic Properties: λmax 289 nm, ε 12.5 L mmol-1 cm-1 (Tris-HCl pH 7.5)

Applications:
Incorporation into DNA/cDNA by

  • PCR with Taq polymerase in-house data

Description:
5-DBCO-PEG4-dUTP is recommended for two-step labeling of DNA/cDNA e.g. by PCR. It is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dTTP. The resulting DBCO-functionalized DNA/cDNA can subsequently be labeled via Cu(I)-free click chemistry that offers the choice

  • to introduce a Biotin group (via Azides of Biotin) for subsequent purification tasks
  • to introduce fluorescent group (via Azides of fluorescent dyes) for subsequent microscopic imaging
  • to crosslink the DNA to Azide-functionalized biomolecules e.g.proteins