Biotin-11-ddCTP
![Structural formula of Biotin-11-ddCTP (Biotin-X-5-Propargylamino-ddCTP, γ-[N-(Biotin-6-amino-hexanoyl)]-5-propargylamino-2',3'-dideoxycytidine-5'-triphosphate, Triethylammonium salt)](/image/ViewImageFit:1731x1299://images/Structures/NU-850-BIOX.png "product Biotin-11-ddCTP")
For research use only.
Please centrifuge briefly before opening (volume ≤2 ml).
Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C28H44N7O15P3S (free acid)
Molecular Weight: 843.67 g/mol (free acid)
Exact Mass: 843.18 g/mol (free acid)
Purity: ≥ 95 % (HPLC)
Form: filtered solution (30 kDa) in 10 mM Tris-HCl
Color: colorless to slightly yellow
Concentration: 1.0 mM - 1.1 mM
pH: 7.5 ±0.5
Spectroscopic Properties: λmax 294 nm, ε 9.3 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Selected References:
Edwards et al. (2001) DNA sequencing using biotinylated dideoxynucleotides and mass spectrometry. Nucleic Acids Res. 29 (21):E104.
| Cat. No. | Amount | Price |
|---|---|---|
| NU-850-BIOX-S | 25 μl (1 mM) | 91,00 € |
| NU-850-BIOX-L | 5 x 25 μl (1 mM) | 333,10 € |
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Scientific citations for Jena Bioscience nu-850-biox
2 scientific citations and 0 citation images found by Bioz.
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Complex genomic patterns of abasic sites in mammalian DNA revealed by a high-resolution SSiNGLe-AP method.
Nature Communications, 2022-10-07 · PMID:36198706
The eluted DNA from above wasmixed with 2 μl of 10× Terminal Transferase Reaction Buffer and 2 μl of 2.5mM CoCl2 and denatured at 95 °C for 30 s followed by
rapid snap-cooling on ice, followed by the addition of 1 nmol biotin-11-ddCTP (Jena Bioscience ,NU-850-BIOX -L -
Simultaneous Determination of Multiple microRNA Levels Utilizing Biotinylated Dideoxynucleotides and Mass Spectrometry.
PLoS ONE, 2017-07-31 · PMID:27380276
SBE reaction mixtures contained 15 μL of clean-up reaction product, 125 pmol each
of biotin11-ddCTP (Jena Biosciences ) and biotin-11-ddGTP (Jena Biosciences), 2 units ThermoPol DNA Polymerase (New England Biolabs), 6 μL of reaction buffer, four SBE primers (10 pmol for miR-31, 30 pmol for miR-24-3p, 40 pmol each for miR-142-3p and the spike-in), and water in a 60 μL volume.
