EDA-GTP-Cy3
excitation and emission spectrum of Cy3
For research use only.
Please centrifuge briefly before opening (volume ≤2 ml).
Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C44H58N9O22P3S2 (free acid)
Molecular Weight: 1222.03 g/mol (free acid)
Exact Mass: 1221.24 g/mol (free acid)
Purity: ≥ 95 % (HPLC)
Form: solution in water
Color: pink
Concentration: 1.0 mM - 1.1 mM
pH: 7.5 ±0.5
Spectroscopic Properties: λexc 550 nm, λem 570 nm, ε 150.0 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Selected References:
Lang et al. (2017) Actin ADP-ribosylation at Threonine148 by Photorhabdus luminescens toxin TccC3 induces aggregation of intracellular F-actin. Cell. Microbiol. 19 (1): e12636.
Anderson et al. (2011) A fluorescent GTP analog as a specific, high-precision label of microtubules. BioTechniques 51 (1):43.
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Scientific citations for Jena Bioscience nu-820-cy3
2 scientific citations and 1 citation images found by Bioz.
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Deciphering the Molecular and Functional Basis of RHOGAP Family Proteins
The Journal of Biological Chemistry, 2016-09-23 · PMID:27481945
Fluorescent Nucleotides Various fluorescence reporter groups, including mant, tamra, and
cy3 , have been coupled to 2′(3′)-hydroxyl group of the ribose moiety of the guanine nucleotide GTP via ethylenediamine to obtain fluorescent GTP variants (Jena Bioscience , Germany) for the analysis of the GAP-stimulated GTP hydrolysis reactions of the RHO proteins. -
A fluorescent GTP analog as a specific, high-precision label of microtubules.
BioTechniques, 2011-11-15 · PMID:21781052
urified from calf brains (29) and stored in 0.5-mg aliquots in 0.2 mM GTP at -80°C. Tubulin, GTP, and either TAMRA-EDAGTP, Cy3-EDA-GTP, or Cy5-EDA-GTP (NU-820-TAM,
NU-820-CY3 , NU-820CY5, respectively;Jena Bioscience , Jena, Germany), hereafter referred to as fluorescent GTP, were mixed with glycerol microtubule buffer (MT buffer: 80 mM PIPES, 10 mM MgCl2, 1 mM EGTA, 30% v/v glycerol, pH 7.0) to typ
