2'/3'-O-(2-Aminoethyl-carbamoyl)-Guanosine-5'-[(β,γ)-imido] triphosphate, labeled with ATTO 488, Triethylammonium salt
For research use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 6 months after date of delivery
Molecular Formula: C38H44N11O23P3S2 (free acid)
Molecular Weight: 1179.87 g/mol (free acid)
Purity: ≥ 95 % (HPLC)
Form: yellow solution in water
Concentration: 1.0 mM - 1.1 mM
pH: 7.5 ±0.5
Spectroscopic Properties: λexc 500 nm, λem 520 nm, ε 90.0 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Please note: For reasons of stability, please make sure that the pH value of a solution of this product never drops below 7.0. This can be achieved by dissolving the nucleotide in a buffer of your choice (50 - 100 mM, pH 7 - 10). Dissolve and adjust concentration photometrically.
Lee et al. (2017) Mechanism of SOS PR-domain autoinhibition revealed by single-molecule assays on native protein from lysate. Nat. Commun. 8:15061.
Christensen et al. (2016) One-way membrane trafficking of SOS in receptor-triggered Ras activation. Nat. Struct. Mol. Biol. 23 (9):838.
Lin et al. (2014) H-Ras forms dimers on membrane surfaces via a protein-protein interface. Proc. Natl. Acad. Sci. USA 111 (8):2996.