7-Propargylamino-7-deaza-dATP-Cy3

excitation and emission spectrum of Cy3

For general laboratory use.

Please centrifuge briefly before opening (volume ≤2 ml).

Shipping: shipped on gel packs

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Shelf Life: 12 months after date of delivery

Molecular Formula: C₄₅H₅₆N₇O₁₉P₃S₂ (free acid)

Molecular Weight: 1156.01 g/mol (free acid)

Exact Mass: 1155.23 g/mol (free acid)

Purity: ≥ 95 % (HPLC)

Form: solution in 10 mM Tris-HCl

Color: pink

Concentration: 1.0 mM - 1.1 mM

pH: 7.5 ±0.5

Spectroscopic Properties: λ_exc 550 nm, λ_em 570 nm, ε 150.0 L mmol^-1 cm^-1 (Tris-HCl pH 7.5)

Applications:
Incorporation into DNA/cDNA by

• Primer Extension with Klenow fragment^[1]

BIOZ Product Citations:

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Selected References:
[1] Walsh et al. (2017) Measurement of incorporation kinetics of non-fluorescent native nucleotides by DNA polymerases using fluorescence microscopy. Nucleic Acids Res. 45 (21):e175.