Mus musculus, recombinant
For general laboratory use.
Unit Definition: One unit of the protein inhibits the activity of 5 ng RNase A by 50 %. Inhibitor activity is assayed in: 40 mM Tris-HCl (pH 8.0, 25 °C), 10 mM MgSO4, 1 mM CaCl2.
Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Concentration: 40 units/μl
RNase inhibitor should be applied in a working concentration of 40 units (1 μl) per 50 μl reaction volume. The application of RNase inhibitor is recommended for:
RNase inhibitor is a recombinant protein which completely inhibits a broad spectrum of eukaryotic RNases, including RNase A, B and C. It inhibits RNases by binding noncovalently in a 1:1 ratio with high affinity (4 x 10-14M). It does not inhibit the RNases I, T1, T2, H, U1, U2 and Cl3. In addition, RNase inhibitor shows no inhibition of polymerase or reverse transcriptase activity and so can be used for cDNA synthesis and in one-step RT-PCR reactions. The murine version of RNase inhibitor lacks the pair of cysteines identified in human version,therefore it has significantly improved resistance to oxidation.
40 unita/μl RNase Inhibitor in 40 mM HEPES-KOH pH 7.5, 100 mM KCl, 8 mM DTT and 50 % [v/v] glycerol)
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