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λDNA/ HindIII Digest


Cat. No. Amount Price (EUR) Buy / Note
M-101S 100 μg23,70 Add to Basket/Quote Add to Notepad
M-101L 5 x 100 μg95,40 Add to Basket/Quote Add to Notepad
1 μg loaded onto 1.0 % agarose
1 μg loaded onto 1.0 % agarose

For general laboratory use.

Shipping: shipped on gel packs

Storage Conditions: store at -20 °C
Short term storage (up to 1 month) at 4 °C possible.

Shelf Life: 12 months

Form: liquid, DNA fragment mix in 10 mM Tris-HCl pH 7.5, 10 mM EDTA, 8 % (w/v) Glycerol, Tartrazine and Xylene Cyanol FF

Color: green

Concentration: 100 ng/μl

The HindIII digest of λ DNA yields the following 8 discrete fragments (in base pairs): 23130*, 9416, 6557, 4361*, 2322, 2027, 564, 125.

* The cohesive ends (12b cos site of bacteriophage lambda) of fragments 23130 bp and 4361 bp may be separated by heating to 65 °C for 5 min.

Dilute in TE or other buffer of minimal ionic strength. DNA may denature if diluted in dH2O and subsequently heated.

Recommended Load:
5 μl per lane

Lambda DNA (48.502 bp) was completely digested by HindIII, phenol extracted, ethanol precipitated, dissolved in 10 mM Tris-HCl (pH 8.0) and 1 mM EDTA.

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