For general laboratory use.
Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
Short term storage (up to 1 month) at 4 °C possible.
Shelf Life: 12 months
Form: liquid, DNA fragment mix in 10 mM Tris-HCl pH 7.5, 10 mM EDTA, 8 % (w/v) Glycerol, Tartrazine and Xylene Cyanol FF
Concentration: 100 ng/μl
The HindIII digest of λ DNA yields the following 8 discrete fragments (in base pairs): 23130*, 9416, 6557, 4361*, 2322, 2027, 564, 125.
* The cohesive ends (12b cos site of bacteriophage lambda) of fragments 23130 bp and 4361 bp may be separated by heating to 65 °C for 5 min.
Dilute in TE or other buffer of minimal ionic strength. DNA may denature if diluted in dH2O and subsequently heated.
5 μl per lane
Lambda DNA (48.502 bp) was completely digested by HindIII, phenol extracted, ethanol precipitated, dissolved in 10 mM Tris-HCl (pH 8.0) and 1 mM EDTA.
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