BssHII
For research use only.
Please centrifuge briefly before opening (volume ≤2 ml).
Unit Definition: One unit is the amount of enzyme required to completely digest 1 μg of Lambda DNA in 1 hour in a total reaction volume of 50 μl. Enzyme activity was determined in the recommended reaction buffer.
Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Form: liquid (Supplied in 10 mM Tris-HCl pH 7.5, 50 mM KCl, 0.1 mM EDTA, 1 mM DTT, 500 μg/ml BSA, 0.2% [v/v] Triton X-100 and 50 % [v/v] glycerol)
Source: Bacillus stearothermophilus H3
Supplied with: 10x Universal Buffer (UB)
Recommended 50 μl assay
| 5 μl | 10x Universal Buffer (UB) |
|---|---|
| 1 μg | pure DNA1 or PCR product2 |
| 5 units | enzyme |
| fill up to 50 μl | PCR grade water |
1 Supercoiled or high molecular weight DNA (e.g. plant genomic DNA) may require longer incubation time or higher amount of enzyme.
2 Some enzymes may require additional DNA bases flanking the restriction site for complete digestion.
Protocol:
- The enzyme should not exceed 10 % of total reaction volume.
- Add enzyme as last component. Mix components well before adding enzyme. After enzyme addition, mix gently by pipetting. Do not vortex.
- Incubate 60 min. at 50 °C.
- Stop reaction by alternatively:
- Addition of 2.1 μl EDTA pH 8.0 [0.5 M], final 20 mM
- Heat Inactivation (20 min. at 80 °C)
- Spin Column DNA Purification (e.g. PCR Purification Kit, Cat.-No. PP-201S/L)
- Gel Electrophoresis and Single Band Excision (e.g. Agarose Gel Extraction Kit, Cat.-No. PP-202S/L)
- Phenol-Chloroform Extraction or Ethanol Precipitation.
Double Digestion - Buffer Compatibility:
B1 - 100 % Relative Activity*
B2 - 100 % Relative Activity
B3 - 100 % Relative Activity
B5 - 100 % Relative Activity*
1x UB - 100 % Relative Activity (recommended)
* The enzyme exhibits star activity under certain conditions.
Please note that the optimum digestion condition for this enzyme is 1x UB. Within the Universal Buffer (UB) system, the most majority of our enzymes display 100% Relative Activity in 1x UB and only few either in 0.5x or 2x UB. If optimum condition for second enzyme is different than the recommended for the first enzyme, we suggest carrying out first the restriction at the higher recommended concentration of UB and dilute the reaction volume to the adequate UB concentration for further proceeding with the second restriction.
Reaction Enzymes Buffer Guide:
| Buffer 1 | 10 × B1 | 100 mM 100 mM 1000 μg/ml | Tris-HCl (pH 7.9, 25°C) MgCl2 BSA |
|---|---|---|---|
| Buffer 2 | 10 × B2 | 100 mM 100 mM 500 mM 1000 μg/ml | Tris-HCl (pH 7.9, 25°C) MgCl2 NaCl BSA |
| Buffer 3 | 10 × B3 | 500 mM 100 mM 1000 mM 1000 μg/ml | Tris-HCl (pH 7.9, 25°C) MgCl2 NaCl BSA |
| Buffer 4 | 10 × B4 | 100 mM 100 mM 1500 mM 1000 μg/ml | Tris-HCl (pH 7.9, 25°C) MgCl2 NaCl BSA |
| Buffer 5 | 10 × B5 | 200 mM 100 mM 500 mM 1000 μg/ml | Tris-acetate (pH 7.9, 25°C) Mg-acetate K-acetate BSA |
Reaction Buffer Compatibility:
Our restriction enzymes are fully compatible to restrictases and buffer systems from other manufacturers and can be used along in double digestions. To obtain best results, consult the corresponding manuals of all involved products.
DNA Methylation:
No Inhibition: dam, dcm, EcoKI
Inhibition (Blocked): CpG
Quality Control:
All preparations are assayed for contaminating endonuclease, 3'-exonuclease, 5' exonuclease/ 5' phosphatase, as well as nonspecific single- and doublestranded DNase activities.
Related products: Universal Restriction Buffer, #EN-300
| Cat. No. | Amount | Price |
|---|---|---|
| EN-E2115-01 | 200 Units | 57,30 € |
| EN-E2115-02 | 5 x 200 Units | 226,60 € |
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Scientific citations for Jena Bioscience en-e2115
2 scientific citations and 0 citation images found by Bioz.
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Quantifying the effect of magnesium ions on nucleosome stability in chromatin fibers
Biophysical Journal, 2026-04-07 · PMID:41793002
Template DNA for chromatin reconstitution was linearized with
BssHII (10 U/μg ), and the 5′ overhangs were filled with dGTP and biotin-16-dCTP using Klenow fragment (Jena Bioscience ) according to the manufacturer’s instructions.. Linearization with BssHII yields DNA handles approximately equal in size (∼4.1 and 4.8 kb), .. -
Identification of essential cis element in 5'UTR of Nef mRNA for Nef translation.
Current HIV research, 2015-04-01 · PMID:24981044
Abstract: Nef is one of the accessory proteins of the human immunodeficiency virus type 1 (HIV-1). Nef is translated from multiple-spliced mRNAs transcribed from the viral genome, whose mRNAs have a relatively long 5' untranslated region (5'UTR). Here, we identified a cis element in the 5'UTR of Nef mRNA essential for efficient Nef translation, which was named the Nef-translation essential region (NER)
