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Molecular Biology

Restriction Enzymes: "B" Enzymes

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BamHI EN-103
JBSpeed Restriction Enzyme

5'–G↓GATC C–3'

3'–C CTAG↑G–5'

BanII EN-E2060
Isoschizomers: Eco24I, EcoT38I, FriOI
JBSpeed Restriction Enzyme

5'–G RGCY↓C–3'

3'–C↑YCGR G–5'

BclI EN-104
Isoschizomers: FbaI, Ksp22I
JBSpeed Restriction Enzyme

5'–T↓GATC A–3'

3'–A CTAG↑T–5'

5'–GCCN NNN↓NGGC–3'

3'–GCCN↑NNN NGGC–5'

5'–A↓GATC T–3'

3'–T CTAG↑A–5'

BseAI EN-107
Isoschizomers: AccIII, Bsp13I, BspEI, Kpn2I, MroI
JBSpeed Restriction Enzyme

5'–T↓CCGG A–3'

3'–A GGCC↑T–5'

BseBI EN-108
Isoschizomers: BstNI, BstOI, Bst2UI, MvaI,
Neoschizomers: AjnI, EcoRII, Psp6I, PspGI
JBSpeed Restriction Enzyme

5'–CC↓W GG–3'

3'–GG W↑CC–5'

BseCI EN-109
Isoschizomers: BanIII, Bsa29I, Bsp106I, BspDI, BspXI, Bsu15I, BsuTUI, ClaI, ZhoI
JBSpeed Restriction Enzyme

5'–AT↓CG AT–3'

3'–TA GC↑TA–5'

BshFI EN-110
Isoschizomers: BspANI, BsuRI, HaeIII, PalI, PhoI
JBSpeed Restriction Enzyme

5'–GG↓CC–3'

3'–CC↑GG–5'

BssHII EN-E2115
Isoschizomers: BsePI, PauI

5'–G↓CGCG C –3'

3'–C GCGC↑G–5'

BstEII EN-144
Isoschizomers: BstPI, Eco91I, EcoO65I, PspEI

5'–G↓GTNAC C–3'

3'–C CANTG↑G–5'

BstXI EN-E2118
JBSpeed Restriction Enzyme

5'–CCAN NNNN↓NTGG–3'

3'–GGTN↑NNNN NACC–5'

Universal Restriction Buffer EN-300
10 x conc.

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