Universal Restriction Buffer
For general laboratory use.
Please centrifuge briefly before opening (volume ≤2 ml).
Description:
Universal Restriction Buffer is a single buffer system optimized for fast and efficient digestion fitting to a broad range of restriction enzymes. The proprietary composition ensures highest enzymatic activity without the need for additional optimization steps.
Shipping: shipped at ambient temperature
Storage Conditions: store at 4 °C or -20 °C
Shelf Life: 12 months
Form: liquid
Concentration: 10x
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Scientific citations for Jena Bioscience en-300
10 scientific citations and 1 citation images found by Bioz.
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Reduced ATP turnover during hibernation in relaxed skeletal muscle
Nature Communications, 2025-01-02 · PMID:39747078
The fiber was incubated in a
rigor buffer solution (potassium acetate120 mM , MOPS 50 mM, EGTA 4 mM, potassium dihydrogen phosphate 5 mM, magnesium acetate 5 mM, DTT 1 mM, pH 6.8) for 5 min. Then, the fiber was moved to another chamber containing rigor buffer plus 250 μM mantATP (NU-202L,Jena Bioscience , Germany) and incubated for 10 min. At the beginning of the recording, the fiber was moved to a new chamber containing fresh relaxing buffer (rigor buffer with the addition of 4 mM ATP). -
Phosphorylation of P-stalk proteins defines the ribosomal state for interaction with auxiliary protein factors.
EMBO Reports, 2024-10-28 · PMID:39468350
.. Mini EDTA-free Protease Inhibitor Cocktail), frozen in liquid nitrogen, and preserved at −70 °C.. The following day, the frozen cells were ground with corundum (Merck, Sigma-Aldrich cat#8334) in
buffer A supplemented with DNase I (Jena Bioscience , cat#EN173S).. The obtained cell lysate was centrifuged at 105,000 × g (MPW-150R, MPW Med Instruments) .. -
The Feline Calicivirus Leader of the Capsid Protein Has the Functional Characteristics of a Viroporin
Viruses, 2022-03-18 · PMID:35337042
To obtain pRSETA-LC and pRSETA-LC-C40A prokaryotic expression plasmids, WT-LC and mutant-LC-C40A codifying sequences were obtained from Wt-LC-pAm-Cyan and Mut-LC-pAm-Cyan vectors previously, respectively [ ], through plasmid digestion with XhoI (
Jena Bioscience , Jena, Germany ) and HindIII (New England Biolabs, Ipswich, MA, USA) restriction enzymes using theJenas Bioscience Universal Buffer™ and were purified from agarose gels with the QIAGEN (Hilden, Germany) purification kit, following the manufacturer’s instructions.
