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Fluorescent Long Range DNA Ladder

100 bp to 10 kb linear scale

ready-to-use, orange / blue, green fluorescent

Cat. No. Amount Price (EUR) Buy / Note
M-225S 500 μl (205 ng/μl) 78,93 Add to Basket/Quote Add to Notepad
M-225L 5 x 500 μl (205 ng/μl) 315,70 Add to Basket/Quote Add to Notepad
5 μl loaded onto 1 % agarose
5 μl loaded onto 1 % agarose

For in vitro use only!

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C
store dark
Short term storage (up to 1 month) at 4 °C possible.

Shelf Life: 12 months

Form: liquid, green, DNA fragment mix in 10 mM Tris-HCl pH 7.5, 10 mM EDTA, 10 % (w/v) Glycerol, EvaGreenTM, Orange G and Xylene Cyanol FF

Concentration: 205 ng/μl

Description:
Fluorescent Long Range DNA Ladder consists of 19 DNA fragments ranging from 100 bp to 10 kb. It is designed to show virtually uniform spacing over an extreme wide fragment range covering virtually the complete application spectrum of DNA ladders. The ladder allows sizing and concentration estimate of DNA fragments on agarose gels generated by PCR or restriction digest. The prestained fragment mix is supplied in ready-to-use format containing EvaGreenTM fluorescent DNA stain and orange / blue tracking dyes.

Fluorescent DNA Stain:
DNA Ladders with Fluorescent Stain contain EvaGreenTM a fluorescent DNA intercalator dye specially developed for DNA analysis applications. High quantum yield and excellent stability makes EvaGreenTM the ideal fluorophore for DNA staining applications and a superior replacement for the widely used dyes Ethidium Bromide or SYBR® Green.
DNA Ladders with Fluorescent Stain are optimized for direct loading into unstained agarose gels and are recommended for use in combination with Gel Loading Buffer with DNA Stain (#PCR-274 - #PCR-276).
The ladders provide highest convenience during routine handling and avoid commonly used gel staining procedures with Ethidium Bromide or SYBR® Green.

Recommended Load:
5 μl per lane

DNA Fragments:
100, 200, 300, 400, 500, 600, 700, 800, 900 bp,
1.0, 1.2, 1.5, 2.0, 3.0, 4.0, 5.0, 6.0, 8.0, 10.0 kb