Despite the variety, many scientists choose their well-known PCR mixes for routine applications without questioning. Those were recommended by fellow lab members or identified in test series some years ago. But are they still the gold standard today?
Routine applications in particular, which are carried out frequently or at large scales should be revised according to the current state of science and product developments reguarly. Challenge your day-to-day routines and test our PCR mixes against your own gold standard.
Check out ready-to-use Crystal Taq Master (Fig. 1A), ideal mix for routine PCR, high throughput PCR and genotyping. Our Ruby Taq Master (Fig. 1B) contains additional gel loading buffer and inherent red dye. This allows easy visual control during PCR set-up and direct loading of the reaction product into the gel. Both mixes are available also with Hot Start Polymerase.
* Amplification of 345 bp hIR (human Insulin Receptor) gene fragment tested as template dilution series (5 - 50 pg), reaction: 96 °C, 2 min; 35x (96 °C, 20 sec; 54 °C, 20 sec; 72 °C, 30 sec), 1 kb DNA Ladder
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