» Sign in / Register

Ruby Hot Start Master (2x)

Red master mix for highly sensitive and specific PCR, direct gel loading

Ready-to-Use Mixes for PCR

Cat. No. Amount Price (EUR) Buy / Note
PCR-165S 4 x 1,25 ml (2x conc.)163,40 Add to Basket/Quote Add to Notepad
PCR-165L 20 x 1.25 ml (2x conc.)653,30 Add to Basket/Quote Add to Notepad
PCR-165XL 100 ml (2x conc.)2.090,50 Add to Basket/Quote Add to Notepad

For general laboratory use.

Shipping: shipped on gel packs

Storage Conditions: store at -20 °C
Short term storage (up to 3 month) at 4 °C possible.

Shelf Life: 12 months

Form: liquid

Concentration: 2x conc.

Ruby Hot Start Master is a 2 x conc. ready-to-use master mix with blocked polymerase activity at ambient temperature.
The master mix is recommended for routine PCR applications, high throughput PCR or genotyping and provides an improved specificity and sensitivity when amplifying low-copy-number targets, working with complex backgrounds or when prolonged room-temperature set-ups are unavoidable.
The thermal activation at the onset of the initial denaturation prevents the extension of nonspecifically annealed primers and primer-dimer formation at low temperatures during PCR setup.
It contains all reagents required for PCR (except template and primer) in a well-balanced ratio to ensure high specificity and minimal by-product formation in almost all PCR applications without the need of additional optimization steps.
Ruby Hot Start Master contains an inherent red dye and gel loading buffer allowing an easy visual control during PCR set-up and the direct loading of the reaction product into the gel.
The mix guarantees robust and reliable amplification results with a minimum of pipetting steps, saves time and reduces the risk of contaminations.
The total PCR assay volume is freely adaptable to individual protocols or the requirements of automated pipetting systems.


Cat.No.Master MixPCR-grade waterAssays x 50 μl
PCR-165S4 x 1.25 ml6 ml200
PCR-165L20 x 1.25 ml2 x 12 ml1000
PCR-165XL100 ml100 ml4000

2 x concentrated PCR master mix containing aptamer inhibited hot start Taq polymerase, nucleotides (dATP, dCTP, dGTP, dTTP), KCl, (NH4)2SO4, MgCl2, red dye, density reagent, enhancing and stabilizing additives.

Recommended PCR assay:
Before starting, vortex the master mix thoroughly to assure homogeneity.

componentstock. conc.20 μl
50 μl
final conc.
Ruby Hot Start Master2x10 μl25μl1x
Primer Mix
or each primer
10 μM each primer0.4-0.8 μl1-2 μl200-400 nM each primer
Template/ sample DNA < 10 ng < 20 ng
PCR-grade water fill up to 20 μlfill up to 50 μl

Recommended cycling conditions:
Before cycling, vortex PCR tubes or plates to assure homogeneity and centrifuge briefly to remove bubbles.

95 °C2 min1x
95 °C
50 - 68 °C
72 °C
10 - 20 sec
10 - 20 sec
20 sec - 4 min

25 - 30x

1)The annealing temperature depends on the melting temperature of the primers used.
2)The elongation time depends on the length of the fragments to be amplified. A time of 1 min/kb is recommended.

BIOZ Product Citations: