Ruby Taq Master (2x)
For general laboratory use.
Please centrifuge briefly before opening (volume ≤2 ml).
Description:
Ruby PCR Master is a 2 x conc. ready-to-use master mix recommended for routine PCR applications (up to 4 kb fragment length), high throughput PCR or genotyping.
It contains all reagents required for PCR (except template and primer) in a well-balanced ratio to ensure high specificity and minimal by-product formation in almost all PCR applications without the need of additional optimization steps.
Ruby PCR Master contains an inherent red dye and gel loading buffer allowing an easy visual control during PCR set-up and the direct loading of the reaction product into the gel.
The mix guarantees robust and reliable amplification results with a minimum of pipetting steps, saves time and reduces the risk of contaminations.
The total PCR assay volume is freely adaptable to individual protocols or the requirements of automated pipetting systems.
Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
Short term storage (up to 3 month) at 4 °C possible.
Shelf Life: 12 months
Form: liquid
Concentration: 2x conc.
Content:
| Cat.No. | Master Mix | PCR-grade water | Assays x 50 μl |
|---|---|---|---|
| PCR-164S | 4 x 1.25 ml | 6 ml | 200 |
| PCR-164L | 20 x 1.25 ml | 2 x 12 ml | 1000 |
| PCR-164XL | 100 ml | 100 ml | 4000 |
2 x concentrated PCR master mix containing Taq polymerase, nucleotides (dATP, dCTP, dGTP, dTTP), KCl, (NH4)2SO4, MgCl2, red dye, density reagent, enhancing and stabilizing additives.
Recommended PCR assay:
Before starting, vortex the master mix thoroughly to assure homogeneity.
| component | stock. conc. | 20 μl assay | 50 μl assay | final conc. |
|---|---|---|---|---|
| Ruby PCR Master | 2x | 10 μl | 25μl | 1x |
| Primer Mix or each primer | 10 μM each primer | 0.4-0.8 μl | 1-2 μl | 200-400 nM each primer |
| Template/ sample DNA | < 10 ng | < 20 ng | ||
| PCR-grade water | fill up to 20 μl | fill up to 50 μl |
Recommended cycling conditions:
Before cycling, vortex PCR tubes or plates to assure homogeneity and centrifuge briefly to remove bubbles.
| Initial denaturation | 95 °C | 2 min | 1x |
|---|---|---|---|
| Denaturation Annealing1) Elongation2) | 95 °C 50 - 68 °C 72 °C | 10 - 20 sec 10 - 20 sec 20 sec - 4 min | 25 - 30x |
1)The annealing temperature depends on the melting temperature of the primers used.
2)The elongation time depends on the length of the fragments to be amplified. A time of 1 min/kb is recommended.
| Cat. No. | Amount | Price |
|---|---|---|
| PCR-164S | 4 x 1,25 ml (2x conc.) | 83,40 € |
| PCR-164L | 20 x 1.25 ml (2x conc.) | 333,60 € |
| PCR-164XL | 100 ml (2x conc.) | 1.000,80 € |
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Scientific citations for Jena Bioscience pcr-164
16 scientific citations and 0 citation images found by Bioz.
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Two worlds beneath: Distinct microbial strategies of the rock-attached and planktonic subsurface biosphere.
Microbiome, 2026-01-31 · PMID:41620752
.. rock material yielded no product at this stage and were hence not considered further.. Obtained amplicons were barcoded in a second PCR as previously described [23], using 1 μL of first-round product, 0.5 μM of each barcoding primer, and 2×
Ruby Taq Master Mix (Jena Bioscience , Germany).. This step involved 6 cycles of 95 °C for 45 s, 55 °C for .. -
Anticancer and Antioxidant Activities of Rhizospheric Soil Bacteria of Lophocereus marginatus
International Journal of Microbiology, 2025-01-01 · DOI:10.1155/ijm/1349429
.. and 1392R (5 ′-GGTTACCTTGTTACGACTT-3 ′) [ ] in a T100 thermal cycler (Bio-Rad, Singapore).. PCR was performed in a volume of
50 μ L , using theRuby Taq master mix 2X (Jena Bioscience , Jena, Germany).. The PCR conditions consisted of an initial temperature of 95°C for 2 mins; 25 .. -
Antimicrobial resistance and prevalence of Listeria species from raw milk and dairy products in Burdur, Turkey
Veterinary Medicine and Science, 2024-07-25 · DOI:10.1002/vms3.1551
.. primer sizes and PCR conditions are given in Table .. The following protocol was used to perform simplex PCR on a total volume of 20 μL: In a PCR tube, we combined
10 μL of 2X PCR master mix (Ruby Tag Master, Jena Bioscience,PCR‐164L ), 3 μL of the DNA template, 0.5 μL of each primer (F or R) and 6 μL of nuclease‐free water.. In multiplex PCRs, nuclease‐free water was reduced by the amount ..
