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5-Ethynyl-cytidine (5-EC)

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CLK-087 10 mg 134,80 Add to Basket/Quote Add to Notepad
Structural formula of 5-Ethynyl-cytidine (5-EC)
Structural formula of 5-Ethynyl-cytidine (5-EC)

For general laboratory use.

Shipping: shipped at ambient temperature

Storage Conditions: store at -20 °C
store dry and under inert gas
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Shelf Life: 12 months after date of delivery

Molecular Formula: C11H13N3O5

Molecular Weight: 267.24 g/mol

Exact Mass: 267.09 g/mol

CAS#: 65223-78-1

Purity: ≥ 99 % (HPLC)

Form: solid

Color: white to off-white

Solubility: DMSO

Spectroscopic Properties: λmax 294 nm, ε 8.5 L mmol-1 cm-1 (Tris-HCl pH 7.5)

RNA synthesis monitoring[1]

Ethynyl-labeled cytidine (5-EC) can be used as a replacement for BrU (5-Bromo-uridine) to measure de novo RNA synthesis in proliferating cells. 5-EC is cell permeable and incorporates into nascent RNA instead of its natural analog cytidine.

The resulting ethynyl-functionalized RNA can subsequently be detected via Cu(I)-catalyzed click chemistry that offers the choice to introduce a Biotin group (via Azides of Biotin) for subsequent purification tasks or a fluorescent group (via Azides of fluorescent dyes) for subsequent microscopic imaging[1].

Presolski et al.[2] and Hong et al.[3] provide a general protocol for Cu(I)-catalyzed click chemistry reactions that may be used as a starting point for the set up and optimization of individual assays.

BIOZ Product Citations:

Selected References:
[1] Qu et al. (2013) 5-Ethynylcytidine as a new agent for detecting RNA synthesis in live cells by click chemistry. Anal. Biochem. 434:128.