The relationship between chromatin structure and genome activity is essential for understanding genomic processes such as transcription and replication. Among the existing chromosome confirmation capture (3C) techniques, Hi-C has become the method of choice for structural characterization of the entire genome both on cell population and single-cell level[2-5]. More information...
A key-step of Hi-C experiments relies on efficient biotinylation of digested chromatin fragments that is performed via incorporation of Biotin-14-dCTP or Biotin-14-dATP by DNA polymerase I (Klenow fragment) (Fig.1).
Figure 1: Efficient biotinylation of digested chromatin fragments is a key-step of Hi-C experiments. Adapted from .
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