The relationship between chromatin structure and genome activity is essential for understanding genomic processes such as transcription and replication. Among the existing chromosome confirmation capture (3C) techniques[1], Hi-C has become the method of choice for structural characterization of the entire genome both on cell population and single-cell level[2-5]. More information...
A key-step of Hi-C experiments relies on efficient biotinylation of digested chromatin fragments that is performed via incorporation of Biotin-14-dCTP[2] or Biotin-14-dATP[3] by DNA polymerase I (Klenow fragment) (Fig.1).
Figure 1: Efficient biotinylation of digested chromatin fragments is a key-step of Hi-C experiments. Adapted from [2].
Compound | Purity | Amount | Price (€) |
---|---|---|---|
Biotin-14-dATP | >/= 95 % (HPLC-MS) | 10 µl, 1 mM | 92,25 |
5x 10 µl, 1 mM | 282,50 | ||
Biotin-14-dCTP | >/= 95 % (HPLC-MS) | 10 µl, 1 mM | 92,25 |
5x 10 µl, 1 mM | 282,50 |
[1] de Wit et al. (2012) A decade of 3C technologies: insights into nuclear organization. Genes Dev. 26 (1):11.
[2] Lieberman-Aiden et al. (2009) Comprehensive mapping of long range interactions reveals folding principles of the human genome. Science 326 (5959):289.
[3] Nagano et al. (2015) Single-cell Hi-C for genome-wide detection of chromatin interactions that occur simultaneously in a single cell. Nature Protocols 10 (12):1987.
[4] Belaghzal et al. (2017) Hi-C 2.0: An optimized Hi-C procedure for high-resolution genome-wide mapping of chromosome confirmation. Methods 123:56.
[5] Berkum et al. (2010) Hi-C: A Method to Study the Three-dimensional Architecture of Genomes. J. Vis. Exp. 39:1869.