rat lung, recombinant, E. coli
| Cat. No. | Amount | Price (EUR) | Buy / Note |
|---|---|---|---|
| PCR-399-1KU | 1 kilo unit | 29,70 | Add to Basket/Quote Add to Notepad |
| PCR-399-10KU | 10 kilo units | 237,60 | Add to Basket/Quote Add to Notepad |
| PCR-399-100KU | 100 kilo units | 1.900,80 | Add to Basket/Quote Add to Notepad |
For general laboratory use.
Please centrifuge briefly before opening (volume ≤2 ml).
Unit Definition: One unit is defined as the amount of RNase inhibitor required to inhibit the activity of 5 ng of ribonuclease A by 50 %.
Shipping: shipped on gel packs
Storage Conditions: store at -80 °C
avoid freeze/thaw cycles
storage at -20 °C for 6 months possible
Shelf Life: see Certificate of Analysis, stored at -80 °C
6 months from delivery date if stored at -20 °C
Form: liquid
Concentration: 40 units/μl
Applications:
RNase Inhibitor should be applied in a working concentration of 10-20 units per 20 μl reaction volume. The application of RNase Inhibitor is recommended for:
Description:
RNase Inhibitor - glycerol-free is recommended for use in freeze drying applications where glycerol must be avoided.
RNase Inhibitor, a recombinant protein of rat lung RNase Inhibitor expressed in E. coli , effectively inhibits a broad spectrum of eukaryotic RNases, including RNase A, B and T2. It inhibits RNases by binding noncovalently in a 1:1 ratio with high affinity (4 x 10-14M). In addition, RNase Inhibitor shows no inhibition of DNA polymerase or reverse transcriptase activity and is used in cDNA synthesis and in one-step RT-PCR reactions for protection against RNase contamination.
Content:
40 units/μl RNase Inhibitor in 20 mM HEPES-KOH pH 7.6, 50 mM KCl,
8 mM DTT, 0.1 % Tween-20
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