Toxoplasma Gondii Dense Granule Protein 7
Toxoplasma gondii, recombinant, E. coli
For in vitro use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Purity: > 95 % (SDS-PAGE)
Form: liquid (Supplied in 50 mM Tris-HCl pH 8.0, 1.5 M urea and 50% glycerol)
The protein contains the p29 (GRA7) immunodominant regions, amino acids 24-100. The protein is purified by proprietary chromatographic technique.
Background: Toxoplasma gondii is an obligate intracellular protozoan parasite that infects all warmblooded animals, including humans, and causes toxoplasmosis. GRA7 is a recently discovered 29-kDa protein. Like GRA1, it is secreted from the dense granules, and it reacts with sera from humans with acute and chronic infections.
Antigen in ELISA and Western blots, excellent antigen for detection of Toxoplasma gondii with minimal specificity problems.
Specificity: Immunoreactive with sera of T. gondii- infected individuals.
Hiszczynska-Sawicka et al. (2003) High yield expression and singlestep purification of Toxoplasma gondii SAG1, GRA1, and GRA7 antigens in Escherichia coli. Protein Expr. Purif. 27:150.
Neudeck et al. (2002) Expression variance, biochemical and immunological properties of Toxoplasma gondii dense granule protein GRA7. Microbes Infect. 4:581.
Vercammen et al. (2000) DNA vaccination with genes encoding Toxoplasma gondii antigens GRA1, GRA7, and ROP2 induces partially protective immunity against lethal challenge in mice. Infect. Immun. 68:38.
Ferguson et al. (1999) In vivo expression and distribution of dense granule protein 7 (GRA7) in the exoenteric (tachyzoite, bradyzoite) and enteric (coccidian) forms of Toxoplasma gondii. Parasitology. 119:259.
Jacobs et al. (1999) Evaluation of recombinant dense granule antigen 7 (GRA7) of Toxoplasma gondii for detection of immunoglobulin G antibodies and analysis of a major antigenic domain. Clin. Diagn. Lab. Immunol. 6:24.
Bonhomme et al. (1998) Quantitative immunolocalization of a P29 protein (GRA7), a new antigen of toxoplasma gondii. J. Histochem. Cytochem. 46:1411.