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(Desthio)Biotinylated Nucleotides for RNA Labeling


Nucleotide Selector for RNA Labeling
Select a suitable nucleotide substrate for enzymatic RNA Labeling.

Table 1: Enzymatic incorporation of Biotinylated Nucleotides


cRNA Labeling
by in vitro Transcription with...
3’-RNA Labeling by... 5’-RNA Labeling
by in vitro Transcription with...
Nucleotide Cat. No. T7 RNA Polymerase T3 RNA Polymerase SP6 RNA Polymerase PAP T4 RNA Ligase T7 RNA Polymerase
UTP
Biotin-11-UTP NU-821-BIOX 1 2 2 - - -
Biotin-16-UTP NU-821-BIO16 1 2 2 - - -
Desthiobiotin-11-UTP NU-821-Desthiobio 1 n/a n/a - - -
CTP
Biotin-11-CTP NU-831-BIOX 1 2 n/a - - -
Desthiobiotin-11-CTP NU-831-Desthiobio 1 n/a n/a - - -
ATP
Biotin-11-ATP NU-957-BIOX 1 n/a n/a n/a - -
GTP
Biotin-11-GTP NU-971-BIOX 1 n/a n/a - - -
AMP
Biotin-AMP NU-894-BIO - - - - - 1
Nucleotide-5’/3’-phosphate
pCp-Biotin NU-1706-BIO - - - - 2 -
pCp-Desthiobiotin NU-1706-Desthiobio - - - - 2 -
5’-Biotin-ApG NU-888-BIO - - - - - 2
yPAP: Yeast Poly A Polymerase
TdT: Terminal deoxynucleotidyl Transferase.
1: in-house tested
2: external reference (see datasheet)
n/a: no data available
- : not applicable