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Biotinylated Nucleotides for RNA Labeling


Nucleotide Selector for DNA/RNA Labeling
Select a suitable nucleotide substrate for enzymatic DNA/RNA Labeling.

Table 1: Enzymatic incorporation of Biotinylated Nucleotides


cRNA Labeling
by in vitro Transcription with...
3’-RNA Labeling by...5’-RNA Labeling
by in vitro Transcription with...
NucleotideCat. No.T7 RNA Polymerase T3 RNA PolymeraseSP6 RNA PolymerasePAPT4 RNA LigaseT7 RNA Polymerase
UTP
Biotin-11-UTPNU-821-BIOX222n/a--
Biotin-16-UTPNU-821-BIO16222n/a--
CTP
Biotin-11-CTPNU-831-BIOX22n/an/a --
ATP
Biotin-7-ATP
N6 position
NU-805-BIOn/an/an/a2--
Biotin-11-ATP
7-Deaza position
NU-957-BIOX2n/an/an/a--
GTP
Biotin-11-GTPNU-971-BIOX2n/an/an/a--
AMP
Biotin-AMPNU-894-BIO-----2
Nucleotide-5’/3’-phosphate
pCp-BiotinNU-1706-BIO----2-
pCp-DesthiobiotinNU-1706-Desthiobio----2-
5’-Biotin-ApGNU-888-BIO-----2
yPAP: Yeast Poly A Polymerase
TdT: Terminal deoxynucleotidyl Transferase.
1: in-house tested
2: external reference (see datasheet)
n/a: no data available
- : not applicable