CLICK-functionalized Nucleotides for RNA Labeling

Nucleotide Selector for RNA Labeling
Select a suitable nucleotide substrate for enzymatic RNA Labeling.

Table 1: Enzymatic incorporation of CLICK-functionalized nucleotides.

		cRNA Labeling by in vitro Transcription with...			3'-RNA Labeling by...
Nucleotide	Cat. No.	T7 RNA Polymerase	T3 RNA Polymerase	SP6 RNA Polymerase	yPAP	TdT	T4 RNA Ligase
UTP
5-Azidomethyl-UTP	CLK-083	✔¹	n/a	n/a	n/a	n/a	n/a
5-Azido-C₃-UTP	NU-157	✔^1,2	n/a	n/a	-	-	-
5-Azido-PEG₄-UTP	CLK-054	✔¹	n/a	n/a	n/a	n/a	n/a
5-Ethynyl-UTP	CLK-T08	✔¹	n/a	n/a	-	-	-
DBCO-PEG₄-UTP	CLK-055	✔¹	n/a	n/a	n/a	n/a	n/a
Vinyl-UTP	CLK-069	✔¹	n/a	n/a	n/a	n/a	n/a
ATP
8-Azido-ATP	NU-155	-	-	-	✔²	-	-
3'-Azido-2',3'-ddATP	NU-882	-	-	-	✔²	-	-
CTP
5-Azido-PEG₄-CTP	CLK-053	✔¹	n/a	n/a	n/a	n/a	n/a
5-DBCO-PEG₄-CTP	CLK-056	✔¹	n/a	n/a	n/a	n/a	n/a
3'-dATP (Cordycepin derivative)
N⁶-Azidohexyl-3'-dATP	NU-1707	-	-	-	n/a	-	-
Cytidine-5'/3'-phosphate
pCp-Azide	NU-1708	-	-	-	-	-	✔²
CpG
5-DBCO-PEG₄-dCpG	CLK-062	n/a	n/a	n/a	n/a	n/a	n/a

yPAP: Yeast Poly A Polymerase
TdT: Terminal deoxynucleotidyl Transferase.
¹: in-house tested
²: external reference (see datasheet)
n/a: no data available
- : not applicable

Click Chemistry background information

CLICK-functionalized Nucleotides for RNA Labeling

Table 1: Enzymatic incorporation of CLICK-functionalized nucleotides.

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