An easy and cost-efficient method for the preparation of 5-(hydroxy)methylated DNA fragments relies on 5-hydroxmethyl-dCTP (5-hmdCTP) or 5-methyl-dCTP (5-mdCTP) that is enzymatically incorporated into DNA with Taq polymerase instead of its natural counterpart dCTP.
5-(hydrox)ymethylated DNA fragments can subsequently be used as sequencing control [1-5] or for pull-down of 5-hydroxymethyl-binding proteins from cellular lysate.
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 Szwagierczak et al. (2011) Characterization of PvuRts1I endonuclease as a tool to investigate genomic 5-hydroxymethylcytosine. Nucl. Acids Res. 39(12):5149.
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 Lafaye et al. (2014) DNA binding of the p21 repressor ZBTB2 is inhibited by cytosine hydroxymethylation. Biochem. Biophys. Res. Commun. 446:341.