(dUMPNPP)
2'-Deoxyuridine-5'-[(α,β)-imido]triphosphate, Sodium salt
| Cat. No. | Amount | Price (EUR) | Buy / Note |
|---|---|---|---|
| NU-903S | 50 μl (10 mM) | 165,50 | Add to Basket/Quote Add to Notepad |
| NU-903L | 5 x 50 μl (10 mM) | 484,70 | Add to Basket/Quote Add to Notepad |
![Structural formula of dUpNHpp ((dUMPNPP), 2'-Deoxyuridine-5'-[(α,β)-imido]triphosphate, Sodium salt)](/images/Structures/NU-903.png)
For general laboratory use.
Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C9H16N3O13P3 (free acid)
Molecular Weight: 467.15 g/mol (free acid)
Exact Mass: 466.99 g/mol (free acid)
CAS#: 170428-86-1
Purity: ≥ 95 % (HPLC)
Form: solution in water
Color: colorless to slightly yellow
Concentration: 10 mM - 11 mM
pH: 7.5 ±0.5
Spectroscopic Properties: λmax 262 nm, ε 10.2 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Applications:
X-ray analysis of vaccinia virus dUTPase[1]
X-ray analysis of DNA-polymerase β[2]
Kinetic of dUTPase[3]
Inhibition of dUTPase[4]
Specific Ligands:
Vaccine virus dUTPase[1]
DNA-polymerase β[2]
BIOZ Product Citations:
Selected References:
[1] Samal et al. (2007) Structures of vaccinia virus dUTPase and ist nucleotide complexes. Acta crystallographica Section D D63:571.
[2] Batra et al. (2006) Magnesium-induced assembly of a complex DNA polymerase catalytic complex. Structure 14:757.
[3] Tóth et al. (2007) Kinetic Mechanism of Human dUTPase, an Essential Nucleotide Pyrophosphatase Enzyme. The journal of bioogical chemistry 282 (46):33572.
[4] Persson et al. (1996) Synthesis of 2'-deoxyuridine 5'- (alpha,beta-imido)triphosphate: A substrate analogue and potent inhibitor of dUTPase. Bioorgan. Med. Chem. 4 (4):553.
Xia et al. (2011) Structural insights into complete metal ion coordination from ternary complexes of B family RB69 DNA polymerase. Biochemistry. 50 (42): 9114.
Badalucco et al. (2011) Crystallization of Chlorella deoxyuridine triphosphatase. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 67 (Pt 12): 1599.
Pecsi et al. (2010) Aromatic stacking between nucleobase and enzyme promotes phosphate ester hydrolysis in dUTPase. Nucleic Acids Res. 38 (20):7179.
Siggaard et al. (2009) Concerted bifunctionality of the dCTP deaminase-dUTPase from Methanocaldococcus jannaschii: A structural and pre-steady state kinetic analysis. Archives of Biochemistry and Biophysics 490 (1):42.
Varga et al. (2008) Active site of mycobacterial dUTPase: Structural characteristics and a built-in sensor. Biochemical and Biophysical Research Communications 373:8.
Vertessy et al. (2007) Flexible segments modulate co-folding of dUTPase and nucleocapsid proteins. Nucleic Acids Research 35 (2):495.
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