2'-Deoxythymidine-5'-[(α,β)-methyleno]triphosphate, Sodium salt
For research use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C11H19N2O13P3 (free acid)
Molecular Weight: 480.20 g/mol (free acid)
Purity: ≥ 95 % (HPLC)
Form: clear aqueous solution
Concentration: 10 mM - 11 mM
pH: 7.5 ±0.5
Spectroscopic Properties: λmax 267 nm, ε 9.6 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Gowda et al. (2012) Low fidelity bypass of O(2)-(3-pyridyl)-4-oxobutylthymine, the most persistent bulky adduct produced by the tobacco specific nitrosamine 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone by model DNA polymerases. Chem. Res. Toxicol. 25 (6):1195.
Beese et al. (1993) Crystal structures of the Klenow fragment of DNA polymerase I complexed with deoxynucleoside triphosphate and pyrophosphate. Biochemistry 32 (51):14095.