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In vitro synthesis of RNA is catalyzed by bacteriophage (SP6, T7 or T3) RNA polymerases using linear DNA as a template. Ribonucleoside triphosphates (NTPs) are the building blocks of these in vitro transcription → Molecular Biology reactions.
Since amplification sensitivity, product yield and reproducibility are strongly affected by contaminating impurities, the quality of NTPs is of considerably importance.
Each lot of Jena Bioscience's ultrapure (> 99 %) NTPs is confirmed free of DNase, RNase, Protease and nicking activity. Furthermore, all NTPs are functionally tested by T7 RNA Polymerase-mediated in vitro transcription.
If you require large amounts (> 100 ml, > 1 l), additional bulk discounts are available. Please contact us at nucleotides@jenabioscience.com to receive an individual quotation.