dApCpp

For general laboratory use.

Shipping: shipped on gel packs

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Shelf Life: 12 months after date of delivery

Molecular Formula: C₁₁H₁₈N₅O₁₁P₃ (free acid)

Molecular Weight: 489.21 g/mol (free acid)

Exact Mass: 489.02 g/mol (free acid)

CAS#: 138998-91-1

Purity: ≥ 95 % (HPLC)

Form: solution in water

Color: colorless to slightly yellow

Concentration: 10 mM - 11 mM

pH: 7.5 ±0.5

Spectroscopic Properties: λ_max 259 nm, ε 15.4 L mmol^-1 cm^-1 (Tris-HCl pH 7.5)

Applications:
X-ray analysis^{[1, 2]}

Specific Ligands:

DNA-polymerase β^[2]

BIOZ Product Citations:

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Selected References:
[1] Batra et al. (2008) Structure of DNA polymerase β with active-site mismatches suggest a transient abasic site intermediate during misincorporation. Molecular Cell 30:315.
[2] Bakhtina et al. (2009) Contribution of the Reverse Rate of the Conformational Step to Polymerase # Fidelity. Biochemistry 48 (14):3197.
Kirby et al. (2012) Metal-Induced DNA Translocation Leads to DNA Polymerase Conformational Activation. Nucleic Acids Res. 40 (7):2974.
Jiang et al. (2011) Use of Chromophoric Ligands to Visually UNIT 7.15 Screen Co-Crystals of Putative Protein-Nucleic Acid Complexes. Curr. Protoc. Nucleic Acid Chem. 7 (7):15.1
Balbo et al. (2011) Kinetic mechanism of active site assembly and chemical catalysis of DNA polymerase β.Biochemistry 50 (45):9865.