dGTP - Solution

For general laboratory use.

Please centrifuge briefly before opening (volume ≤2 ml).

Description:
dGTP, PCR-grade is supplied as ultrapure aqueous solution (pH 8.5) and suitable for all molecular biology applications including PCR/qPCR, reverse transcription, DNA labeling and DNA sequencing.

Shipping: shipped on gel packs

Storage Conditions: store at -20 °C

Shelf Life: 12 months

Molecular Formula: C₁₀H₁₆N₅O₁₃P₃ (free acid)

Molecular Weight: 507.18 g/mol (free acid)

CAS#: 93919-41-6

Purity: ≥ 99 % (HPLC)

Form: clear aqueous solution

Concentration: 100 mM - 110 mM

pH: 8.5 ±0.2 (22 °C)

Spectroscopic Properties: λ_max 252 nm, ε 14.2 L mmol^-1 cm^-1 (Tris-HCl pH 7.0)

Selected References:
Holland et al. (1991) Detection of specific polymerase chain reaction product by utilizing the 5'----3' exonuclease activity of Thermus aquaticus DNA polymerase. Proc. Natl. Acad. Sci. USA 88 (16):7276.
Erlich et al. (1988) Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 29 (239):487.
Sanger et al. (1977) DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463.