Reference dye for fluorescence signal normalization
For general laboratory use.
Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
Shelf Life: 12 months
Form: liquid, supplied in 20 mM Tris-HCl pH 8.5, 0.1 mM EDTA and 0.01 % Tween-20.
Concentration: 25 μM (50x conc.)
Spectroscopic Properties: λexc 576 nm, λem 601 nm
ROX reference dye is optimized for block-based real-time PCR instruments that are compatible with the evaluation of the ROX reference signal. The dye does not take part in the PCR reaction but allows to normalize for non-PCR related signal variation and provides a baseline in multiplex reactions. The dye is supplied as 50x concentration.
Preparation of the qPCR master mix:
Preparation of a master mix is crucial in quantitative PCR reactions to reduce pipetting errors. Vortex the ROX reference dye thoroughly before use. Add ROX reference dye to your PCR assay as indicated below.
|20 μl PCR assay||50 μl PCR assay|
|low ROX assay|
|0.04 μl||0.1 μl|
|high ROX assay|
|0.4 μl||1.0 μl|
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