500 bp to 10 kb linear scale
ready-to-use, blue / blue, green fluorescent
For general laboratory use.
Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
Short term storage (up to 1 month) at 4 °C possible.
Shelf Life: 12 months
Form: liquid, DNA fragment mix in 10 mM Tris-HCl pH 7.5, 10 mM EDTA, 10 % (w/v) Glycerol, SYBR® Green, Bromophenol Blue and Xylene Cyanol FF
Concentration: 130 ng/μl
Fluorescent High Range DNA Ladder consists of 12 DNA fragments ranging from 500 bp to 10 kb. It is designed to show virtually uniform spacing over a wide fragment range. The ladder allows sizing and concentration estimate of DNA fragments on agarose gels generated by PCR or restriction digest. The prestained fragment mix is supplied in ready-to-use format containing SYBR® Green fluorescent DNA stain and blue tracking dyes.
Fluorescent DNA Stain:
DNA Ladders with Fluorescent Stain contain SYBR® Green a fluorescent DNA intercalator dye specially developed for DNA analysis applications. High quantum yield and excellent stability makes SYBR® Green the ideal fluorophore for DNA staining applications and a superior replacement for Ethidium Bromide.
DNA Ladders with Fluorescent Stain are optimized for direct loading into unstained agarose gels and are recommended for use in combination with Gel Loading Buffer with DNA Stain (#PCR-274 - #PCR-276).
The ladders provide highest convenience during routine handling and avoid commonly used gel stains like Ethidium Bromide.
5 μl per lane
0.5, 0.6, 0.8, 1, 1.5, 2, 3, 4, 5, 6, 8, 10 kb
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