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Green Gel Loading Buffer with DNA Stain

Loading buffer for agarose or polyacrylamide gels with SYBR® Green fluorescent DNA stain

Cat. No. Amount Price (EUR) Buy / Note
PCR-275 5 x 1,8 ml 66,90 Add to Basket/Quote Add to Notepad

For general laboratory use.

Shipping: shipped on gel packs

Storage Conditions: store at -20 °C
store dark

Shelf Life: 12 months

Form: Liquid

Concentration: 6x conc.

Green Gel Loading buffer with DNA Stain is recommended for analyzing DNA fragments 100 - 2000 bp.

Add one part of Gel Loading Buffer to 5 parts of the DNA sample as shown in the table below. Spin down the tubes and vortex gently to achieve homogeneity.

DNA sample5 μl10 μl20 μl50 μl
6x Gel Loading Buffer1 μl2 μl4 μl10 μl

Jena Bioscience Gel Loading Buffers with DNA Stain are formulated to facilitate loading of DNA samples into the wells of agarose and polyacrylamide gels. The loading buffers contain SYBR® Green DNA Stain a fluorescent DNA intercalator dye specially developed for DNA analysis applications. High quantum yield and excellent stability makes SYBR® Green the ideal fluorophore for DNA staining applications and a superior replacement for the widely used dyes Ethidium Bromide.
The buffers contain tracking dyes as indicator for DNA fragment migration. In addition, they contain glycerol to add density and EDTA to inhibit nuclease activities.
The buffers are optimized for loading of DNA fragments in a size range of:

Gel Loading BufferCat. No.fragment size
Blue Gel Loading BufferPCR-274larger than 500 bp
Green Gel Loading BufferPCR-275from 100 to 2000 bp
Orange Gel Loading BufferPCR-276smaller than 500 bp

6x Gel Loading Buffer:
60 mM Tris-HCl (pH 7.5), 60 mM EDTA, 50 % (w/v) Glycerol, xylene cyanol FF, Tartrazine, SYBR® Green

The Gel Loading Buffer provides highest convenience during routine handling and avoids commonly used gel staining procedures with Ethidium Bromide.

Fragment Separation on Agarose Gels:

DNA fragment sizerecommended agarose gel conc.xylene cyanol FF running at approx.bromo-
phenol blue running
at approx.
orange G running at approx.
20-400 bp3.6 %280 bp40 bp2 bp
50-1000 bp3.0 %500 bp60 bp2 bp
100-2000 bp2.4 %900 bp100 bp3 bp
200-4000 bp1.8 %1800 bp40 bp5 bp
0.5-10 kb1.2 %4.5 kb0.5 kb10 bp
1-30 kb0.6 %12 kb1.2 kb100 bp

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