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Poly (A) carrier RNA: Improved viral RNA recovery

Poly (A) carrier RNA improves recovery of low RNA amounts presumably by enhanced aggregation.

Most commercial viral RNA purification kits therefore recommend the addition of Poly (A) carrier RNA for efficient purification from biological fluids which usually contain limited viral RNA amounts only.

Poly (A) carrier RNA-purified viral RNA is compatible with subsequent downstream analyses such as (RT-)qPCR or digital droplet PCR (ddPCR).

High-quality Poly (A) carrier RNA is now available:

Product Cat.-No. Amount Price
Poly (A) carrier RNA RNT-017-S 100 mg 149 €
RNT-017-L 1 g 995 €
Parameter Specification
Appearance Lyophilized white to off-white solid
Poly(A) in relation to mononucleotide unit (A257*, ɛ = 10700 M-1cm-1) 2.3 - 2.5 µmol/mg
chain length
(agarose gelelectrophoresis)
2100 to 10000 nucleotides
A250/260* 0.82 - 0.92
A280/260* 0.25 - 0.35
A290/260* 0.03 - 0.05
RNAse/DNAse contamination
(FRET assay)
not detectable
* absorbance measurement in DNAse/RNAse free water

Questions or inquiries?

Dr. Barbara Zschoernig

Please contact Barbara with all questions or inquiries you may have!