Poly (A) carrier RNA improves recovery of low RNA amounts presumably by enhanced aggregation.
Most commercial viral RNA purification kits therefore recommend the addition of Poly (A) carrier RNA for efficient purification from biological fluids which usually contain limited viral RNA amounts only.
Poly (A) carrier RNA-purified viral RNA is compatible with subsequent downstream analyses such as (RT-)qPCR or digital droplet PCR (ddPCR).
|Poly (A) carrier RNA||RNT-017-S||100 mg||149 €|
|RNT-017-L||1 g||995 €|
|Appearance||Lyophilized white to off-white solid|
|Poly(A) in relation to mononucleotide unit (A257*, ɛ = 10700 M-1cm-1)||2.3 - 2.5 µmol/mg|
|2100 to 10000 nucleotides|
|A250/260*||0.82 - 0.92|
|A280/260*||0.25 - 0.35|
|A290/260*||0.03 - 0.05|
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