Polyadenylic acid, potassium salt
For in vitro use only!
Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible. Store dark.
Shelf Life: 12 months
Molecular Weight: 100 – 500 kDa
Form: lyophilized solid
Color: white to off-white
Poly (A) carrier RNA is used for quantitative precipitation/purification of RNA and DNA. It improves recovery of short fragments (< 200 bp) or low amounts of nucleic acids.
Chain length: 2100 to 10000 nucleotides
Recommended working concentration: 0.5 mg/ml
Poly (A) in relation to one mononucleotide unit:
2.3 - 2.5 μmol/mg (A257*, ɛ = 10.7 L mmol-1 cm-1)
A290/A260*: 0.03 - 0.05
A280/A260*: 0.25 - 0.35
A250/A260*: 0.82 - 0.92
DNAse/RNAse contamination: not detectable (FRET assay)
*absorbance measurement in DNAse/RNAse free water