Poly (A) carrier RNA

For general laboratory use.

Shipping: shipped on gel packs

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible. Store dark.

Shelf Life: 12 months

Molecular Weight: 100 – 500 kDa

Form: lyophilised solid

Color: white to off-white

Description:
Poly (A) carrier RNA is used for quantitative precipitation/purification of RNA and DNA. It improves recovery of short fragments (< 200 bp) or low amounts of nucleic acids.

Chain length: 2100 to 10000 nucleotides

Recommended working concentration: 0.5 mg/ml

Quality control:
Poly (A) in relation to one mononucleotide unit:
2.3 - 2.5 μmol/mg (A₂₅₇*, ɛ = 10.7 L mmol^-1 cm^-1)
A₂₉₀/A₂₆₀*: 0.03 - 0.05
A₂₈₀/A₂₆₀*: 0.25 - 0.35
A₂₅₀/A₂₆₀*: 0.82 - 0.92
DNAse/RNAse contamination: not detectable (FRET assay)

^*absorbance measurement in DNAse/RNAse free water

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