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AF-Dyes (also known as Alexa Fluor® dyes)

Traditional dyes such as fluorescein or TAMRA are often hampered by low photostability and/or low hydrophilicity. Both however are critical parameter for detection sensitivity and bioconjugate solubility, respectively.

AF-Dyes (also known as Alexa Fluor® dyes) are hydrophilic fluorescent dyes with remarkable photostability. We now offer both functionalized AF-Dyes and AF-Dye – nucleotide conjugates. CLICK AF-Dyes are routinely used for Click Chemistry – based labeling whereas NHS Ester or Maleimide-functionalized AF-Dyes react with primary amine or thiol modified biomolecules, respectively.


CLICK AF-Dyes Non-CLICK AF-Dyes AF-Dye - Nucleotide conjugates
Dye Substitute
for
Excmax
[nm]**
Emmax
[nm]**
εmax* Azide Picolyl-Azide Alkyne DBCO***
= ADIBO/DIBAC
NHS Ester Maleimide dUTP
AF488
(also known as Alexa Fluor®488)
Cy2
FITC (Fluorescein)
ATTO488
494 517 73
AF546
(also known as Alexa Fluor®546)
TAMRA 554 570 110 - - -
AF555
(structural analog to Alexa Fluor®555)
Cy3
ATTO550
555 572 155 +
AF594
(also known as Alexa Fluor®594)
Texas Red 590 617 92
AF647
(structural analog to Alexa Fluor®647)
Cy5
ATTO647N
648 671 270 +
- : not available
+ : available as custom synthesis
* in [L x mmol-1 x cm-1] (free acid)
** free acid
*** DBCO = Dibenzocyclooctyne, ADIBO = Azadibenzocyclooctyne, DIBAC = Dibenzoazacyclooctyne


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