SARS-Associated Coronavirus Envelope
recombinant, E. coli
For in vitro use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Molecular Weight: 43 kDa
Purity: > 95 % (SDS-PAGE)
Form: liquid (Supplied in 50 mM Tris-HCl, 60 mM NaCl and 50% glycerol)
Recombinant SARS-ACE Antigen may be used in ELISA and Western blots, excellent for detection of SARS with minimal specificity problems.
SARS-ACE contains the N-terminus Envelope protein immunodominant regions, amino acids: 1-76. SARS-ACE is purified by proprietary chromatographic techniques.
Background: SARS (Severe Acute Respiratory Syndrome) Coronavirus is an enveloped virus containing three outer structural proteins, namely the membrane (M), envelope (E), and spike (S) proteins. Spike (S)-glycoprotein of the virus interacts with a cellular receptor and mediates membrane fusion to allow viral entry into susceptible target cells. Accordingly, S-protein plays an important role in virus infection cycle and is the primary target of neutralizing antibodies.
Specificity: Immunoreactive with sera of SARS infected individuals.
Liao et al. (2004) Expression of SARS-coronavirus envelope protein in Escherichia coli cells alters membrane permeability. Biochem. Biophys. Res Commun. 325:374.
Shen et al. (2003) Small envelope protein E of SARS: cloning, expression, purification, CD determination, and bioinformatics analysis. Acta Pharmacol. Sin. 24:505.