HTLV-1 Envelope

For general laboratory use.

Shipping: shipped on gel packs

Storage Conditions: store at -20 °C
avoid freeze/thaw cycles

Shelf Life: 12 months

Molecular Weight: 27 kDa

Purity: > 95 % (SDS-PAGE, RP-HPLC)

Form: liquid (Supplied in 10 mM NaPO₄ pH 6.0, 0.1% SDS and 1 mM DTT)

Applications:
Antigen in ELISA and Western Blots. Excellent reagent for correct detection of HTLV infections with minimal specificity problems.

Description:
The protein contains the C- terminus of gp46 and most of p21E of HTLV-1. This non-fusion E. coli derived protein starts from HTLV-1 envelope amino acids 165 and ends with 440. The protein is purified by proprietary chromatographic technique.

Background: Human T-cell leukemia virus (HTLV)-1 and -2 are deltaretroviruses that infect a wide range of cells. HTLV-1 has been found primarily in CD4⁺ and CD8⁺ T-lymphocytes in vivo, whereas CD8⁺ T-lymphocytes are thought to be the in vivo reservoir of HTLV-2.

Specificity: Immunoreactive with all sera of HTLV-1 and HTLV-2 infected individuals with antibody response to HTLV envelope.

BIOZ Product Citations:

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Selected References:
Hernandez et al. (2001) Chimeric synthetic peptides containing two immunodominant epitopes from the envelope gp46 and the transmembrane gp21 glycoproteins of HTLV-I virus. Biochem. Biophys. Res. Commun. 289:1.
Armand et al. (2000) Targeted expression of HTLV-I envelope proteins in muscle by DNA immunization of mice. Vaccine. 18:2212.
Tallet et al. (2000) Expression, purification and biological properties of the carboxyl half part of the HTLV-I surface envelope glycoprotein. J. Chromatogr. B. Biomed. Sci. Appl. 737:85.
Georges-Gobinet et al. (1998) HTLV-I associated sicca syndrome in Guadeloupe: lack of relation with a peculiar encoding sequence of surface envelope glycoprotein. Virus. Genes. 16:195.
Grange et al. (1998) Identification of exposed epitopes on the envelope glycoproteins of human T-cell lymphotropic virus type I (HTLV-I). Int. J. Cancer. 75:804.
Grange et al. (1997) Induction of neutralizing antibodies against HTLV-I envelope proteins after combined genetic and protein immunizations in mice. DNA Cell. Biol. 16:1439.
Kim et al. (2004) HTLV-1 and -2 envelope SU subdomains and critical determinants in receptor binding. Retrovirology. 1:41.