Human Immunodeficiency Virus 1 Trans-Acting Transcription factor
recombinant, E. coli
For in vitro use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Molecular Weight: 14 kDa
Purity: > 90 % (SDS-PAGE, HPLC)
Form: lyophilized (with 0.1% glycerol)
Solubility: It is recommended to reconstitute the lyophilized HIV-1 TAT in bidest H2O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Reacts with anti-Tat polyclonal antibodies from human, monkey, rabbit and mouse serum. Recognized by anti-Tat (HIV-1) polyclonal antibody.
Recombinant HIV-1 TAT produced in E. coli is a single, non-glycosylated, polypeptide chain containing 86 aminoacids encoded by two exons and having chain having a molecular mass of 14 kDa. Recombinant HIV-1 TAT is purified by proprietary chromatographic technique.
Background: HIV belongs to the retrovirus family, distinguished by possession of a viral reverse transcriptase that transcribes viral RNA into DNA which is integrated into the host-cell genome. HIV-1 regulatory Trans-Acting Transcription factor (TAT) plays an essential role in viral replication and infectivity. In addition, during acute infection, TAT is released extracellularly by infected cells and is taken up by neighboring cells where it transactivates viral replication and increases virus infectivity.HIV-1 Tat activates transcription of HIV-1 viral genes by inducing phosphorylation of the C-terminal domain(CTD) of RNA polymerase II.Tat can also disturb cellular metabolism by inhibiting proliferation of antigen-specific T lymphocytes and by inducing cellular apoptosis.
Specificity: Immuno reactive with all sera of HIV-I infected individuals.
Flora et al. (2005) Proinflammatory synergism of ethanol and HIV-1 Tat protein in brain tissue. Exp. Neurol. 191:2.
Partidos et al. (2004) A synthetic HIV-1 Tat protein breaches the skin barrier and elicits Tat-neutralizing antibodies and cellular immunity. Eur. J. Immunol. 34:3723.
Gavioli et al. (2004) HIV-1 tat protein modulates the generation of cytotoxic T cell epitopes by modifying proteasome composition and enzymatic activity. J. Immunol. 173:3838.
Campbell et al. (2004) The glutamine-rich region of the HIV-1 Tat protein is involved in T-cell apoptosis. J. Biol. Chem. 279:48197.
Opi et al. (2004) Full-length HIV-1 Tat protein necessary for a vaccine. Vaccine. 22:3105.
Caputo et al. (2004) Novel biocompatible anionic polymeric microspheres for the delivery of the HIV-1 Tat protein for vaccine application. Vaccine. 22:2910.